ArticleThe Journal of cell biology1996
Metazoan rDNA enhancer acts by making more genes transcriptionally active.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
- Regulation of RNA Polymerase I Stability and Function.Cancers · 2022Review
- A Deconvolution Protocol for ChIP-Seq Reveals Analogous Enhancer Structures on the Mouse and Human Ribosomal RNA Genes.G3 (Bethesda, Md.) · 2018Article
- Article
- Tor pathway regulates Rrn3p-dependent recruitment of yeast RNA polymerase I to the promoter but does not participate in alteration of the number of active genes.Molecular biology of the cell · 2004Article
- In exponentially growing Saccharomyces cerevisiae cells, rRNA synthesis is determined by the summed RNA polymerase I loading rate rather than by the number of active genes.Molecular and cellular biology · 2003Article
- RPD3 is required for the inactivation of yeast ribosomal DNA genes in stationary phase.The EMBO journal · 2002Article
- Positive feedback in eukaryotic gene networks: cell differentiation by graded to binary response conversion.The EMBO journal · 2001Article
- Survey and summary: transcription by RNA polymerases I and III.Nucleic acids research · 2000Review
- Variegated expression of the endogenous immunoglobulin heavy-chain gene in the absence of the intronic locus control region.Molecular and cellular biology · 1999Article
- Transcriptional activity and chromatin structure of enhancer-deleted rRNA genes in Saccharomyces cerevisiae.Molecular and cellular biology · 1999Article
- The interferon-inducible nucleolar p204 protein binds the ribosomal RNA-specific UBF1 transcription factor and inhibits ribosomal RNA transcription.The EMBO journal · 1999Article
- Dimerization and HMG box domains 1-3 present in Xenopus UBF are sufficient for its role in transcriptional enhancement.Nucleic acids research · 1998Article
- Chromatin domains and nuclear compartments: establishing sites of gene expression in eukaryotic nuclei.Molecular biology reports · 1997Review
- Transcription reinitiation rate: a special role for the TATA box.Molecular and cellular biology · 1997Article
- The Xenopus RNA polymerase I transcription factor, UBF, has a role in transcriptional enhancement distinct from that at the promoter.The EMBO journal · 1997Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Enhancers could, in principle, function by increasing the rate of reinitiation on individual adjacent active promoters or by increasing the probability that an adjacent promoter is activated for transcription. We have addressed this issue for the repetitive metazoan rDNA enhancer by microinjecting Xenopus oocytes with enhancer-less and enhancer-bearing genes and determining by EM the frequency that each gene type forms active transcription units and their transcript density. We use conditions where transcription requires the normal rDNA promoter and is stimulated 30-50-fold by the enhancer. (In contrast, at saturating template conditions as used in previous EM studies, an aberrant mode of transcription is activated that is not affected by the rDNA enhancer or by the generally recognized rDNA promoter). The active transcription units on enhancer-less genes are found to be as densely packed with nascent transcripts and polymerases as those on enhancer-bearing genes and on the endogenous rRNA genes. Significantly, the enhancer-bearing genes are approximately 30-50-fold more likely to form such active transcription units than enhancer-less genes, consistent with their amounts of transcript. Complementary studies confirm that the enhancer does not affect elongation rate, the stability of the transcription complex, or transcript half-life. These data demonstrate that the repetitive metazoan rDNA enhancer causes more genes to be actively transcribed and does not alter the reinitiation rate on individual active genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.