ArticleThe Journal of cell biology1996
Demonstration of a dynamic, transcription-dependent organization of pre-mRNA splicing factors in polytene nuclei.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 40 citations in OpenAlex.
- The histone variant H2A.Z promotes efficient cotranscriptional splicing inGenes & development · 2017Article
- Intranuclear binding in space and time of exon junction complex and NXF1 to premRNPs/mRNPs in vivo.The Journal of cell biology · 2015Article
- The Chironomus tentans genome sequence and the organization of the Balbiani ring genes.BMC genomics · 2014Article
- Review
- Acetylation by the transcriptional coactivator Gcn5 plays a novel role in co-transcriptional spliceosome assembly.PLoS genetics · 2009Article
- Splicing factors SF1 and U2AF associate in extraspliceosomal complexes.Molecular and cellular biology · 2008Article
- A specific SR protein binds preferentially to the secretory protein gene transcripts in salivary glands of Chironomus tentans.Chromosoma · 2006Article
- Chironomus tentans-repressor splicing factor represses SR protein function locally on pre-mRNA exons and is displaced at correct splice sites.Molecular biology of the cell · 2006Article
- Poly(A)+ RNAs roam the cell nucleus and pass through speckle domains in transcriptionally active and inactive cells.The Journal of cell biology · 2004Article
- In vivo recruitment of exon junction complex proteins to transcription sites in mammalian cell nuclei.RNA (New York, N.Y.) · 2004Article
- A novel conserved RNA-binding domain protein, RBD-1, is essential for ribosome biogenesis.Molecular biology of the cell · 2002Article
- The mRNA export factor Dbp5 is associated with Balbiani ring mRNP from gene to cytoplasm.The EMBO journal · 2002Article
- In situ transcription and splicing in the Balbiani ring 3 gene.The EMBO journal · 2001Article
- Spatial organization of RNA polymerase II transcription in the nucleus.Nucleic acids research · 2000Review
- Intron-independent association of splicing factors with active genes.The Journal of cell biology · 1999Article
- Inefficient processing impairs release of RNA from the site of transcription.The EMBO journal · 1999Article
- The perinucleolar compartment and transcription.The Journal of cell biology · 1998Article
- Nuclear history of a pre-mRNA determines the translational activity of cytoplasmic mRNA.The EMBO journal · 1998Article
- A nuclear matrix protein interacts with the phosphorylated C-terminal domain of RNA polymerase II.Molecular and cellular biology · 1998Article
- Spliced exons of adenovirus late RNAs colocalize with snRNP in a specific nuclear domain.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe the dynamic organization of pre-mRNA splicing factors in the intact polytene nuclei of the dipteran Chironomus tentans. The snRNPs and an SR non-snRNP splicing factor are present in excess, mainly distributed throughout the interchromatin. Approximately 10% of the U2 snRNP and an SR non-snRNP splicing factor are associated with the chromosomes, highly enriched in active gene loci where they are bound to RNA. We demonstrate that the splicing factors are specifically recruited to a defined gene upon induction of transcription during physiological conditions. Concomitantly, the splicing factors leave gene loci in which transcription is turned off. We also demonstrated that upon general transcription inhibition, the splicing factors redistribute from active gene loci to the interchromatin. Our findings demonstrate the dynamic intranuclear organization of splicing factors and a tight linkage between transcription and the intranuclear organization of the splicing machinery.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.