ArticleProceedings of the National Academy of Sciences of the United States of America1996
Substrate-bound agrin induces expression of acetylcholine receptor epsilon-subunit gene in cultured mammalian muscle cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 83 citations in OpenAlex.
- A link between agrin signalling and CaScientific reports · 2022Article
- Synapse-specific Lrp4 mRNA enrichment requires Lrp4/MuSK signaling, muscle activity and Wnt non-canonical pathway.Cell & bioscience · 2021Article
- Emergence of functional neuromuscular junctions in an engineered, multicellular spinal cord-muscle bioactuator.APL bioengineering · 2020Article
- Extracellular signal-regulated kinases 1 and 2 regulate neuromuscular junction and myofiber phenotypes in mammalian skeletal muscle.Neuroscience letters · 2020Review
- The Structure, Function, and Physiology of the Fetal and Adult Acetylcholine Receptor in Muscle.Frontiers in molecular neuroscience · 2020Review
- Nerve, Muscle, and Synaptogenesis.Cells · 2019Review
- Sema3A Reduces Sprouting of Adult Rod Photoreceptors In Vitro.Investigative ophthalmology & visual science · 2017Article
- CLASP2-dependent microtubule capture at the neuromuscular junction membrane requires LL5β and actin for focal delivery of acetylcholine receptor vesicles.Molecular biology of the cell · 2015Article
- Article
- Acetylcholine receptor (AChR) clustering is regulated both by glycogen synthase kinase 3β (GSK3β)-dependent phosphorylation and the level of CLIP-associated protein 2 (CLASP2) mediating the capture of microtubule plus-ends.The Journal of biological chemistry · 2014Article
- Agrin regulates CLASP2-mediated capture of microtubules at the neuromuscular junction synaptic membrane.The Journal of cell biology · 2012Article
- Soluble miniagrin enhances contractile function of engineered skeletal muscle.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
- Neuregulin/ErbB regulate neuromuscular junction development by phosphorylation of α-dystrobrevin.The Journal of cell biology · 2011Article
- Matrix metalloproteinase-3 in myasthenia gravis compared to other neurological disorders and healthy controls.Autoimmune diseases · 2011Article
- Local induction of acetylcholine receptor clustering in myotube cultures using microfluidic application of agrin.Biophysical journal · 2006Article
- A novel pathway for MuSK to induce key genes in neuromuscular synapse formation.The Journal of cell biology · 2003Article
- Clustering of nicotinic acetylcholine receptors: from the neuromuscular junction to interneuronal synapses.Molecular neurobiology · 2002Review
- Agrin differentially regulates the rates of axonal and dendritic elongation in cultured hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Effects of purified recombinant neural and muscle agrin on skeletal muscle fibers in vivo.The Journal of cell biology · 2001Article
- Src, Fyn, and Yes are not required for neuromuscular synapse formation but are necessary for stabilization of agrin-induced clusters of acetylcholine receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of the epsilon-subunit gene of the acetylcholine receptor (AChR) by myonuclei located at the neuromuscular junction is precisely regulated during development. A key role in this regulation is played by the synaptic portion of the basal lamina, a structure that is also known to contain agrin, a component responsible for the formation of postsynaptic specializations. We tested whether agrin has a function in synaptic AChR gene expression. Synaptic basal lamina from native adult muscle and recombinant agrin bound to various substrates induced in cultured rat myotubes AChR clusters that were colocalized with epsilon-subunit mRNA. Estimation of transcript levels by Northern hybridization analysis of total RNA showed a significant increase when myotubes were grown on substrate impregnated with agrin, but were unchanged when agrin was applied in the medium. The effect was independent of the receptor aggregating activity of the agrin isoform used, and agrin acted, at least in part, at the level of epsilon-subunit gene transcription. These findings are consistent with a role of agrin in the regulation of AChR subunit gene expression at the neuromuscular junction, which would depend on its binding to the synaptic basal lamina.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.