ArticleNucleic acids research1996
Sequence of the polypyrimidine tract of the 3'-terminal 3' splicing signal can affect intron-dependent pre-mRNA processing in vivo.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- SPLICE-q: a Python tool for genome-wide quantification of splicing efficiency.BMC bioinformatics · 2021Article
- An intronic RNA structure modulates expression of the mRNA biogenesis factor Sus1.RNA (New York, N.Y.) · 2016Article
- Binding of hnRNP L to the pre-mRNA processing enhancer of the herpes simplex virus thymidine kinase gene enhances both polyadenylation and nucleocytoplasmic export of intronless mRNAs.Molecular and cellular biology · 2005Article
- A Kaposi's sarcoma virus RNA element that increases the nuclear abundance of intronless transcripts.The EMBO journal · 2005Article
- Pre-mRNA processing enhancer (PPE) elements from intronless genes play additional roles in mRNA biogenesis than do ones from intron-containing genes.Nucleic acids research · 2005Article
- Analysis of the stimulatory effect of splicing on mRNA production and utilization in mammalian cells.RNA (New York, N.Y.) · 2003Article
- A novel function for the U2AF 65 splicing factor in promoting pre-mRNA 3'-end processing.EMBO reports · 2002Article
- Intron presence-absence polymorphism in Drosophila driven by positive Darwinian selection.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Arginine-rich regions mediate the RNA binding and regulatory activities of the protein encoded by the Drosophila melanogaster suppressor of sable gene.Molecular and cellular biology · 2000Article
- Position-dependent inhibition of the cleavage step of pre-mRNA 3'-end processing by U1 snRNP.RNA (New York, N.Y.) · 2000Article
- Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.Microbiology and molecular biology reviews : MMBR · 1999Review
- Intronless mRNA transport elements may affect multiple steps of pre-mRNA processing.The EMBO journal · 1999Article
- Binary specification of nonsense codons by splicing and cytoplasmic translation.The EMBO journal · 1998Article
- Efficient 3'-end formation of human beta-globin mRNA in vivo requires sequences within the last intron but occurs independently of the splicing reaction.Nucleic acids research · 1998Article
- Poly(A)-driven and poly(A)-assisted termination: two different modes of poly(A)-dependent transcription termination.Molecular and cellular biology · 1998Article
- The mouse histone H2a gene contains a small element that facilitates cytoplasmic accumulation of intronless gene transcripts and of unspliced HIV-1-related mRNAs.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Most pre-mRNAs require an intron for efficient processing in higher eukaryotes. However, not all introns can provide this function. For example, transcripts synthesized from a variant of the human beta-globin gene lacking its second intervening sequence (IVS2), yet retaining its first intervening sequence (IVS1), exhibit multiple defects in mRNA biogenesis. To investigate why, we transfected into monkey cells plasmids containing the human beta-globin gene and variants of it altered in (i) IVS1, (ii) the 3'-terminal exon, and (iii) the polyadenylation signal. The beta-globin RNAs accumulated in these cells were analyzed by quantitative S1 nuclease mapping for nuclear accumulation, intron excision, polyadenylation and cytoplasmic accumulation. We found that the 3' splicing signal of IVS1, with multiple purines interrupting its polypyrimidine tract, could efficiently function as an internal 3' splicing signal; however, it could not efficiently function as the 3'-terminal 3' splicing signal for any of these steps in intron-dependent mRNA biogenesis unless (i) its polypyrimidine tract was made uninterrupted in pyrimidines, or (ii) specific sequences were deleted from the 3'-terminal exon. We conclude that whether an intron can provide the function necessary for efficient processing of intron-dependent pre-mRNA is dependent upon the ability of its 3' splicing signal to define the 3'-terminal exon. On the practical side, this finding means one needs to consider both the sequence and location of the intron to be included in an intron-dependent gene to obtain efficient expression in vivo.
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