ArticleMolecular and cellular biology1996
Sp1 binds two sites in the CD11c promoter in vivo specifically in myeloid cells and cooperates with AP1 to activate transcription.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 97 citations in OpenAlex.
- Promoter identification and transcriptional regulation of the metastasis gene MACC1 in colorectal cancer.Molecular oncology · 2013Article
- Regulation of xylosyltransferase I gene expression by interleukin 1β in human primary chondrocyte cells: mechanism and impact on proteoglycan synthesis.The Journal of biological chemistry · 2013Article
- Targeting lentiviral vectors for cancer immunotherapy.Current cancer therapy reviews · 2011Article
- Role for transcription factor TFII-I in the suppression of SSeCKS/Gravin/Akap12 transcription by Src.International journal of cancer · 2011Article
- Rapid generation of dendritic cell specific transgenic mice by lentiviral vectors.Transgenic research · 2009Article
- Sp1 and AP-1 regulate expression of the human gene VIL2 in esophageal carcinoma cells.The Journal of biological chemistry · 2009Article
- Lentiviral vectors for cancer immunotherapy: transforming infectious particles into therapeutics.Gene therapy · 2007Review
- Identification of an histone H3 acetylated/K4-methylated-bound intragenic enhancer regulatory for urokinase receptor expression.Oncogene · 2007Article
- Article
- Direct interaction between FcgammaRI (CD64) and periplakin controls receptor endocytosis and ligand binding capacity.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Molecular characterization of the constitutive expression of the plasma platelet-activating factor acetylhydrolase gene in macrophages.The Biochemical journal · 2003Article
- Isolation and characterization of the human A-myb promoter: regulation by NF-Y and Sp1.Oncogene · 2000Article
- CD43 gene expression is mediated by a nuclear factor which binds pyrimidine-rich single-stranded DNA.Nucleic acids research · 2000Article
- Article
- Characterization of human involucrin promoter distal regulatory region transcriptional activator elements-a role for Sp1 and AP1 binding sites.The Biochemical journal · 1998Article
- Differential transcriptional regulation of the human thrombin receptor gene by the Sp family of transcription factors in human endothelial cells.The Biochemical journal · 1998Article
- Retinoid-induced apoptosis and Sp1 cleavage occur independently of transcription and require caspase activation.Molecular and cellular biology · 1997Article
- CpG islands from the alpha-globin gene cluster increase gene expression in an integration-dependent manner.Molecular and cellular biology · 1997Article
- Sp1 binding plays a critical role in Erb-B2- and v-ras-mediated downregulation of alpha2-integrin expression in human mammary epithelial cells.Molecular and cellular biology · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The leukocyte integrin gene, CD11c, is transcriptionally regulated and is expressed predominantly on differentiated cells of the myelomonocytic lineage. In this study we have demonstrated that the regions -72 to -63 and -132 to -104 of the CD11c promoter contain elements responsible for phorbol ester-induced differentiation of the myeloid cell line HL60. DNase I footprinting analysis revealed that these regions can bind purified Sp1, and supershift analysis with Sp1 antibody confirmed that Sp1 in HL60 nuclear extracts could bind these regions. Transfection analysis of CD11c promoter-chloramphenicol acetyltransferase constructs containing deletions of these Sp1-binding sites revealed that these sites are essential for expression of the CD11c gene in HL60 cells but not in the T-cell line Molt4 or the cervical carcinoma cell line HeLa. Moreover, cotransfection of pPacSp1 along with these CD11c promoter-chloramphenicol acetyltransferase constructs into Sp1-deficient Drosophila Schneider 2 cells verified that these sites are essential for Sp1-dependent expression of the CD11c promoter. In vivo genomic footprinting revealed that Sp1 contacts the CD11c promoter within the regions -69 to -63 and -116 to -105 in phorbol 12-myristate 13-acetate-differentiated HL60 cells but not in undifferentiated HL60 cells or in Molt4 or HeLa cells. Cotransfection assays in HL60 cells revealed that Sp1 acts synergistically with Ap1 to activate CD11c. Further, both Sp1 sites are capable of cooperating with AP1. In vitro DNase I footprinting analysis with purified Sp1 and c-jun proteins showed that Sp1 binding could facilitate binding of c-jun. We propose that myeloid-specific expression of the CD11c promoter and is facilitated by cooperative interaction between the Sp1- and Ap1-binding sites.
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