ArticleMolecular and cellular biology1996
Analysis of mice containing a targeted deletion of beta-globin locus control region 5' hypersensitive site 3.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 175 citations in OpenAlex.
- Evolution of hemoglobin loci and their regulatory elements.Blood cells, molecules & diseases · 2018Review
- Differentiation-Dependent Motility-Responses of Developing Neural Progenitors to Optogenetic Stimulation.Frontiers in cellular neuroscience · 2017Article
- Conditional Creation and Rescue of Nipbl-Deficiency in Mice Reveals Multiple Determinants of Risk for Congenital Heart Defects.PLoS biology · 2016Article
- The Hematopoietic Stem and Progenitor Cell Cistrome: GATA Factor-Dependent cis-Regulatory Mechanisms.Current topics in developmental biology · 2016Review
- A gene expression resource generated by genome-wide lacZ profiling in the mouse.Disease models & mechanisms · 2015Article
- Heterozygosity for deletion of hypersensitive site 3 in the human locus control region has an unexpected minor effect on red cell phenotype.Journal of human genetics · 2014Article
- Hematopoietic transcriptional mechanisms: from locus-specific to genome-wide vantage points.Experimental hematology · 2014Review
- Targeting of Slc25a21 is associated with orofacial defects and otitis media due to disrupted expression of a neighbouring gene.PloS one · 2014Article
- Transcriptional mechanisms underlying hemoglobin synthesis.Cold Spring Harbor perspectives in medicine · 2013Review
- LCR 5' hypersensitive site specificity for globin gene activation within the active chromatin hub.Nucleic acids research · 2012Article
- Deletion of the Scl +19 enhancer increases the blood stem cell compartment without affecting the formation of mature blood lineages.Experimental hematology · 2012Article
- Article
- The higher structure of chromatin in the LCR of the beta-globin locus changes during development.Journal of molecular biology · 2009Article
- Towards a genome-wide reconstruction of cis-regulatory networks in the human genome.Seminars in cell & developmental biology · 2009Review
- Expression of green fluorescent protein under the regulation of human locus control region elements HS2 and HS3 in transgenic mice.International journal of hematology · 2008Article
- Disruption of the C5a receptor gene increases resistance to acute Gram-negative bacteremia and endotoxic shock: opposing roles of C3a and C5a.Molecular immunology · 2008Article
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- Cooperativeness of the higher chromatin structure of the beta-globin locus revealed by the deletion mutations of DNase I hypersensitive site 3 of the LCR.Journal of molecular biology · 2007Article
- Article
- Synergistic and additive properties of the beta-globin locus control region (LCR) revealed by 5'HS3 deletion mutations: implication for LCR chromatin architecture.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
To examine the function of murine beta-globin locus region (LCR) 5' hypersensitive site 3 (HS3) in its native chromosomal context, we deleted this site from the mouse germ line by using homologous recombination techniques. Previous experiments with human 5' HS3 in transgenic models suggested that this site independently contains at least 50% of total LCR activity and that it interacts preferentially with the human gamma-globin genes in embryonic erythroid cells. However, in this study, we demonstrate that deletion of murine 5' HS3 reduces expression of the linked embryonic epsilon y- and beta H 1-globin genes only minimally in yolk sac-derived erythroid cells and reduces output of the linked adult beta (beta major plus beta minor) globin genes by approximately 30% in adult erythrocytes. When the selectable marker PGK-neo cassette was left within the HS3 region of the LCR, a much more severe phenotype was observed at all developmental stages, suggesting that PGK-neo interferes with LCR activity when it is retained within the LCR. Collectively, these results suggest that murine 5' HS3 is not required for globin gene switching; importantly, however, it is required for approximately 30% of the total LCR activity associated with adult beta-globin gene expression in adult erythrocytes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.