ArticleMolecular and cellular biology1996
Signals sufficient for 3'-end formation of yeast mRNA.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 75 citations in OpenAlex.
- Direct RNA-seq provides evidence for an antiterminator function of the yeast Hrp1 protein on RNA polymerase II transcripts.RNA (New York, N.Y.) · 2026Article
- Direct RNA-seq provides evidence for an antiterminator function of the yeast Hrp1 protein on RNA polymerase II transcripts.bioRxiv : the preprint server for biology · 2025Article
- Evolutionary dynamics of polyadenylation signals and their recognition strategies in protists.Genome research · 2024Article
- Delineating yeast cleavage and polyadenylation signals using deep learning.Genome research · 2024Article
- eIF4F complex dynamics are important for the activation of the integrated stress response.Molecular cell · 2024Article
- POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic Biology.ACS synthetic biology · 2024Article
- Development of Terminator-Promoter Bifunctional Elements for Application inInternational journal of molecular sciences · 2023Article
- Review
- Transcriptional neighborhoods regulate transcript isoform lengths and expression levels.Science (New York, N.Y.) · 2022Article
- Effects of sequence motifs in the yeast 3' untranslated region determined from massively parallel assays of random sequences.Genome biology · 2021Article
- Review
- Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex.Molecular cell · 2019Article
- Modeling of Genome-Wide Polyadenylation Signals inFrontiers in genetics · 2019Article
- Protein Abundance Control by Non-coding Antisense Transcription.Cell reports · 2016Article
- Can terminators be used as insulators into yeast synthetic gene circuits?Journal of biological engineering · 2016Article
- Systematic dissection of the sequence determinants of gene 3' end mediated expression control.PLoS genetics · 2015Article
- Isolation and Expression Analysis of CYP9A11 and Cytochrome P450 Reductase Gene in the Beet Armyworm (Lepidoptera: Noctuidae).Journal of insect science (Online) · 2015Article
- Inducible, tightly regulated and growth condition-independent transcription factor in Saccharomyces cerevisiae.Nucleic acids research · 2014Article
- DNA damage induces targeted, genome-wide variation of poly(A) sites in budding yeast.Genome research · 2013Article
- Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications.Metabolic engineering · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The following three elements were previously shown to be required for 3'-end formation of mRNA in the yeast Saccharomyces cerevisiae: (i) the efficiency element TATATA or related sequences, which function by enhancing the efficiency of downstream positioning elements; (ii) the positioning element AATAAA or related sequences, which position the poly(A) site; and (iii) the actual poly(A) site, which is usually Py(A)n. In this study, we synthesized a 39-pb poly(A) signal that contained the optimum sequences of these three elements. By inserting the synthetic 3'-end-forming signal into various positions of a CYC1-lacZ fusion gene, we showed that truncated transcripts of the expected sizes were generated. Furthermore, the poly(A) sites of the truncated transcripts were mapped to the expected poly(A) site within the synthetic signal. Our findings establish that the three elements are not only necessary but also sufficient for mRNA 3'-end formation in S. cerevisiae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.