ArticleJournal of virology1996
Properties of the protein encoded by the UL32 open reading frame of herpes simplex virus 1.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- New Insights into Human Cytomegalovirus pUL52 Structure.Viruses · 2021Article
- A pentameric protein ring with novel architecture is required for herpesviral packaging.eLife · 2021Article
- Kaposi's Sarcoma-Associated Herpesvirus ORF68 Is a DNA Binding Protein Required for Viral Genome Cleavage and Packaging.Journal of virology · 2018Article
- The human cytomegalovirus terminase complex as an antiviral target: a close-up view.FEMS microbiology reviews · 2018Review
- Herpesvirus Capsid Assembly and DNA Packaging.Advances in anatomy, embryology, and cell biology · 2017Review
- The Herpes Simplex Virus Protein pUL31 Escorts Nucleocapsids to Sites of Nuclear Egress, a Process Coordinated by Its N-Terminal Domain.PLoS pathogens · 2015Article
- The putative herpes simplex virus 1 chaperone protein UL32 modulates disulfide bond formation during infection.Journal of virology · 2015Article
- Isolation and characterization of the herpes simplex virus 1 terminase complex.Journal of virology · 2014Article
- Characterization of the subcellular localization of herpes simplex virus type 1 proteins in living cells.Medical microbiology and immunology · 2011Article
- A "coiled-coil" motif is important for oligomerization and DNA binding properties of human cytomegalovirus protein UL77.PloS one · 2011Article
- Characterization of pseudorabies virus (PrV) cleavage-encapsidation proteins and functional complementation of PrV pUL32 by the homologous protein of herpes simplex virus type 1.Journal of virology · 2009Article
- The essential human cytomegalovirus gene UL52 is required for cleavage-packaging of the viral genome.Journal of virology · 2008Article
- The Varicella-zoster virus DNA encapsidation genes: Identification and characterization of the putative terminase subunits.Virus research · 2007Article
- Linker insertion mutations in the herpes simplex virus type 1 UL28 gene: effects on UL28 interaction with UL15 and UL33 and identification of a second-site mutation in the UL15 gene that suppresses a lethal UL28 mutation.Journal of virology · 2006Article
- Herpes simplex virus capsid structure: DNA packaging protein UL25 is located on the external surface of the capsid near the vertices.Journal of virology · 2006Article
- Novel class of thiourea compounds that inhibit herpes simplex virus type 1 DNA cleavage and encapsidation: resistance maps to the UL6 gene.Journal of virology · 2000Article
- Physical and functional interactions between the herpes simplex virus UL15 and UL28 DNA cleavage and packaging proteins.Journal of virology · 1999Article
- Herpes simplex virus type 1 cleavage and packaging proteins UL15 and UL28 are associated with B but not C capsids during packaging.Journal of virology · 1998Article
- The herpes simplex virus type 1 cleavage/packaging protein, UL32, is involved in efficient localization of capsids to replication compartments.Journal of virology · 1998Article
- The null mutant of the U(L)31 gene of herpes simplex virus 1: construction and phenotype in infected cells.Journal of virology · 1997Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The functions previously assigned to the essential herpes simplex virus 1 UL32 protein were in cleavage and/or packaging of viral DNA and in maturation and/or translocation of viral glycoproteins to the plasma membrane. The amino acid sequence predicts N-linked glycosylation sites and sequences conserved in aspartyl proteases and in zinc-binding proteins. We report the following. (i) The 596-amino-acid UL32 protein accumulated predominantly in the cytoplasm of infected cells but was not metabolically labeled with glucosamine and did not band with membranes containing a known glycoprotein in flotation sucrose density gradients. The UL32 protein does not, therefore, have the properties of an intrinsic membrane protein. (ii) Experiments designed to demonstrate aspartyl protease activity in a phage display system failed to reveal proteolytic activity. Moreover, substitution of Asp-110 with Gly in the sequence Asp-Thr-Gly, the hallmark of aspartyl proteases, had no effect on viral replication in Vero and SK-N-SH cell lines or in human foreskin fibroblasts. Therefore, if the UL32 protein functions as a protease, this function is not required in cells in culture. (iii) Both the native UL32 protein and a histidine-tagged UL32 protein made in recombinant baculovirus-infected insect cells bound zinc. The consensus sequence is conserved in the UL32 homologs from varicella-zoster virus and equine herpesvirus 1. UL32 protein is therefore a cysteine-rich, zinc-binding essential cytoplasmic protein whose function is not yet clear.
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