ArticleJournal of virology1996
Genetic analysis of polyomavirus large T nuclear localization: nuclear localization is required for productive association with pRb family members.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Viral infection, APOBEC3 dysregulation, and cancer.Frontiers in genetics · 2024Review
- Nuclear and Nucleolar Localization of Bovine Adenovirus-3 Protein V.Frontiers in microbiology · 2020Article
- Human polyomaviruses and cancer: an overview.Clinics (Sao Paulo, Brazil) · 2018Review
- Identification and characterization of nuclear and nucleolar localization signals in the adeno-associated virus serotype 2 assembly-activating protein.Journal of virology · 2015Article
- Functional ablation of pRb activates Cdk2 and causes antiestrogen resistance in human breast cancer cells.PloS one · 2007Article
- Role of the interaction between large T antigen and Rb family members in the oncogenicity of JC virus.Oncogene · 2006Review
- Activation of CREB/ATF sites by polyomavirus large T antigen.Journal of virology · 2005Article
- Article
- J domain-independent regulation of the Rb family by polyomavirus large T antigen.Journal of virology · 2000Article
- The retinoblastoma protein alters the phosphorylation state of polyomavirus large T antigen in murine cell extracts and inhibits polyomavirus origin DNA replication.Journal of virology · 1999Article
- Polyomavirus large T antigen induces alterations in cytoplasmic signalling pathways involving Shc activation.Journal of virology · 1999Article
- The DnaJ domain of polyomavirus large T antigen is required to regulate Rb family tumor suppressor function.Journal of virology · 1997Article
- Phosphorylation sites in polyomavirus large T antigen that regulate its function in viral, but not cellular, DNA synthesis.Journal of virology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Polyomavirus large T antigen (LT) is a multifunctional nuclear protein. LT has two nuclear localization signals (NLS2), one spanning residues 189 to 195 (NLS1) and another spanning residues 280 to 286 (NLS2). Site-directed mutagenesis showed that each signal contains at least two critical residues. The possibility of connections between NLSs and adjacent phosphorylations has attracted much attention. Cytoplasmic LT (CyT) mutants were underphosphorylated, particularly at sites adjacent to NLS2. However, since a nuclear LT bearing an inactivated NLS2 was phosphorylated normally at adjacent sites, the signal was not directly required for phosphorylation. Conversely, LT could be translocated to the nucleus via NLS2 even when the adjacent phosphorylation sites were deleted. CyT was examined to probe the importance of LT localization. CyT was unable to perform LT functions related to interactions with retinoblastoma susceptibility gene (pRb) family members. Hence, CyT was unable to immortalize primary cells or to transactivate an E2F-responsive promoter. Consistent with these findings, CyT, though capable of binding pRb in vitro, did not cause relocalization of pRb in cells. Assays of transactivation of the simian virus 40 late promoter and of the human c-fos promoter showed that defects of CyT were not limited to functions dependent on pRb interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.