ArticleJournal of virology1996
Nucleoplasmic and nucleolar distribution of the adenovirus IVa2 gene product.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 47 citations in OpenAlex.
- RANBP2 and USP9x regulate nuclear import of adenovirus minor coat protein IIIa.PLoS pathogens · 2022Article
- Article
- Adenovirus Core Proteins: Structure and Function.Viruses · 2021Review
- Article
- Nucleocapsid Interacts with NPM1 and Protects it from Proteolytic Cleavage, Enhancing Cell Survival, and is Involved in PEDV Growth.Scientific reports · 2017Article
- Dynamic Nucleolar Targeting of Dengue Virus Polymerase NS5 in Response to Extracellular pH.Journal of virology · 2016Article
- Components of Adenovirus Genome Packaging.Frontiers in microbiology · 2016Review
- Adenoviral L4 33K forms ring-like oligomers and stimulates ATPase activity of IVa2: implications in viral genome packaging.Frontiers in microbiology · 2015Article
- Identification and characterization of a DNA binding domain on the adenovirus IVa2 protein.Virology · 2012Article
- Nucleolar proteomics and viral infection.Proteomics · 2010Review
- Proteomics analysis of the nucleolus in adenovirus-infected cells.Molecular & cellular proteomics : MCP · 2010Article
- Involvement of the nucleolus in replication of human viruses.Reviews in medical virology · 2009Review
- Adenovirus IVa2 protein binds ATP.Journal of virology · 2008Article
- Relationship between adenovirus DNA replication proteins and nucleolar proteins B23.1 and B23.2.The Journal of general virology · 2007Article
- Identification of a new human adenovirus protein encoded by a novel late l-strand transcription unit.Journal of virology · 2007Article
- A guide to viral inclusions, membrane rearrangements, factories, and viroplasm produced during virus replication.Advances in virus research · 2007Review
- Separate basic region motifs within the adeno-associated virus capsid proteins are essential for infectivity and assembly.Journal of virology · 2006Article
- Dependence of the encapsidation function of the adenovirus L1 52/55-kilodalton protein on its ability to bind the packaging sequence.Journal of virology · 2006Article
- Analysis of the interaction of the adenovirus L1 52/55-kilodalton and IVa2 proteins with the packaging sequence in vivo and in vitro.Journal of virology · 2005Article
- Nuclear localization of the adenovirus E4orf4 protein is mediated through an arginine-rich motif and correlates with cell death.Oncogene · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence elements (DE) located downstream of the adenovirus major late promoter start site have previously been shown to be essential for the activation of this promoter after the onset of viral DNA replication. Two proteins (DEF-A and DEF-B) bind to these elements in a late-phase-dependent manner and contribute to this activation. DEF-B corresponds to a dimer of the adenovirus IVa2 gene product (pIVa2, 449 residues), while DEF-A is a heteromeric protein also comprising pIVa2. As revealed by specific immunofluorescence staining of infected cells, pIVa2 is targeted to the nucleus, where it distributes to both nucleoplasmic and nucleolar structures. We have identified the pIVa2 nuclear localization signal (NLS) as a basic peptide element at the C terminus of the protein (residues 432 to 449). An element essential for nucleolar localization (NuLS) has been mapped in the N-terminal part of pIVa2 (between residues 50 and 136). While NuLS activity is dependent upon an intact NLS, we show that both NLS and NuLS functions are independent of specific DNA-binding activity. As visualized by immunoelectron microscopy, pIVa2 is detected in the nucleoplasm at the level of the fibrillogranular network which is active in viral transcription. More surprisingly, pIVa2 accumulates within electron-dense amorphous inclusions found both in the nucleoplasm and in the nucleolus. Altogether, these results suggest that, besides controlling major late promoter transcription, pIVa2 serves additional, as yet unknown functions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.