ArticleThe Journal of clinical investigation1996
Mechanism of cytokine-induced modulation of beta-adrenoceptor responsiveness in airway smooth muscle.
Article in The Journal of clinical investigation, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed, 132 citations in OpenAlex.
- Unravelling the Role of Post-Junctional M2 Muscarinic Receptors in Cholinergic Nerve-Mediated Contractions of Airway Smooth Muscle.International journal of molecular sciences · 2025Review
- Inhibition of ABCC1 Decreases cAMP Egress and Promotes Human Airway Smooth Muscle Cell Relaxation.American journal of respiratory cell and molecular biology · 2022Article
- Semaphorin3E/plexinD1 Axis in Asthma: What We Know So Far!Advances in experimental medicine and biology · 2021Article
- Mechanisms underlying TNFα-induced enhancement of force generation in airway smooth muscle.Physiological reports · 2019Article
- Drug Repurposing: The Anthelmintics Niclosamide and Nitazoxanide Are Potent TMEM16A Antagonists That Fully Bronchodilate Airways.Frontiers in pharmacology · 2019Article
- Coupling of Airway Smooth Muscle Bitter Taste Receptors to Intracellular Signaling and Relaxation Is via GAmerican journal of respiratory cell and molecular biology · 2017Article
- Long-Term Fluticasone Propionate/Formoterol Fumarate Combination Therapy Is Associated with a Low Incidence of Severe Asthma Exacerbations.Journal of aerosol medicine and pulmonary drug delivery · 2016Article
- Influenza A virus infection and cigarette smoke impair bronchodilator responsiveness to β-adrenoceptor agonists in mouse lung.Clinical science (London, England : 1979) · 2016Article
- Cyclooxygenase-2 and microRNA-155 expression are elevated in asthmatic airway smooth muscle cells.American journal of respiratory cell and molecular biology · 2015Article
- Article
- Targeting α- and β-Adrenergic Receptors Differentially Shifts Th1, Th2, and Inflammatory Cytokine Profiles in Immune Organs to Attenuate Adjuvant Arthritis.Frontiers in immunology · 2014Article
- cAMP regulation of airway smooth muscle function.Pulmonary pharmacology & therapeutics · 2013Review
- A new approach for the study of lung smooth muscle phenotypes and its application in a murine model of allergic airway inflammation.PloS one · 2013Article
- IL-13-induced changes in endogenous glucocorticoid metabolism in the lung regulate the proasthmatic response.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- TNF-α-induces airway hyperresponsiveness to cholinergic stimulation in guinea pig airways.British journal of pharmacology · 2012Article
- G Protein βγ-subunit signaling mediates airway hyperresponsiveness and inflammation in allergic asthma.PloS one · 2012Article
- β2-Agonist induced cAMP is decreased in asthmatic airway smooth muscle due to increased PDE4D.PloS one · 2011Article
- SP-A preserves airway homeostasis during Mycoplasma pneumoniae infection in mice.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
- Activation of endogenous GABAA channels on airway smooth muscle potentiates isoproterenol-mediated relaxation.American journal of physiology. Lung cellular and molecular physiology · 2008Article
- Regulation of heterotrimeric G protein signaling in airway smooth muscle.Proceedings of the American Thoracic Society · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To elucidate the role of specific proinflammatory cytokines in regulating airway responsiveness, we examined the effects and mechanisms of action of IL-1beta, TNF-alpha, and IL-2 on the beta-adrenoceptor- and postreceptor-coupled transmembrane signaling mechanisms regulating relaxation in isolated rabbit tracheal smooth muscle (TSM) segments. During half-maximal isometric contraction of the tissues with acetylcholine, relaxation responses to isoproterenol, PGE2, and forskolin were separately compared in control (untreated) TSM and tissues incubated for 18 h with IL-1beta (10 ng/ml), TNF-(alpha (100 ng/ml), or IL-2 (200 ng/ml). Relative to controls, IL-1beta- and TNF-alpha-treated TSM, but not IL-2-treated tissues, depicted significant attenuation of their maximal relaxation and sensitivity (i.e., -log dose producing 50% maximal relaxation) to isoproterenol (P < 0.001) and PGE2 (P < 0.05); whereas the relaxation responses to direct stimulation of adenylate cyclase with forskolin were similar in the control and cytokine-treated tissues. Further, the attenuated relaxation to isoproterenol and PGE2 was ablated in the IL-1beta-treated TSM that were pretreated with either the muscarinic M2-receptor antagonist, methoctramine (10(-6) M), or pertussis toxin (100 ng/ml). Moreover, Western immunoblot analysis demonstrated that: (a) Gi protein expression was significantly enhanced in membrane fractions isolated from IL-1beta-treated TSM; and (b) the latter was largely attributed to induced enhanced expression of the Gi alpha2 and Gi alpha3 subunits. Collectively, these observations provide new evidence demonstrating that IL-lbeta and TNF-alpha induce impaired receptor-coupled airway relaxation in naive TSM, and that the latter effect is associated with increased muscarinic M2-receptor/Gi protein-coupled expression and function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.