ArticleThe Biochemical journal1996
Transcriptional induction of the human renin gene by cyclic AMP requires cyclic AMP response element-binding protein (CREB) and a factor binding a pituitary-specific trans-acting factor (Pit-1) motif.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 53 citations in OpenAlex.
- Article
- On the top of ARB N/L type Ca channel blocker leads to less elevation of aldosterone.Bioscience reports · 2016Article
- Decidualisation of human endometrial stromal cells is associated with increased expression and secretion of prorenin.Reproductive biology and endocrinology : RB&E · 2015Article
- A polymorphism of the renin gene rs6682082 is associated with essential hypertension risk and blood pressure levels in Korean women.Yonsei medical journal · 2015Article
- A Genetic Variant in the Distal Enhancer Region of the Human Renin Gene Affects Renin Expression.PloS one · 2015Article
- Determinants of plasma renin activity: role of a human renin gene variant as a genetic factor.Medicine · 2014Article
- Control of renin [corrected] gene expression.Pflugers Archiv : European journal of physiology · 2013Review
- cAMP target sequences enhCRE and CNRE sense low-salt intake to increase human renin gene expression in vivo.Pflugers Archiv : European journal of physiology · 2011Article
- Mesenchymal stem cells differentiate into renin-producing juxtaglomerular (JG)-like cells under the control of liver X receptor-alpha.The Journal of biological chemistry · 2010Article
- A single nucleotide mutation in the mouse renin promoter disrupts blood pressure regulation.The Journal of clinical investigation · 2008Article
- Expression of renin in large arteries outside the kidney revealed by human renin promoter/LacZ transgenic mouse.The American journal of pathology · 2002Article
- cAMP increases the expression of human angiotensinogen gene through a combination of cyclic AMP responsive element binding protein and a liver specific transcription factor.Molecular and cellular biochemistry · 2000Article
- Extrapituitary TSH in early chick embryos: Pit-1 dependence?Journal of molecular neuroscience : MNArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To delineate the cis-acting elements of the proximal promoter responsible for cyclic AMP (cAMP)-induced human renin gene transcription, 5'-flanking regions of the human renin gene were fused to a luciferase reporter gene and transfected in chorionic cells. Forskolin treatment induced the expression of luciferase by 2.4-fold when the reporter plasmid contained the promoter region (-582 to + 16). Mutation or deletion of the cAMP response element (CRE) diminished (1.7-fold) but did not abolish cAMP-induced transcription, demonstrating that the (-582 to -145) region containing the CRE and the region (-145 to -38) containing a Pit-1 (pituitary-specific trans-acting factor) site were both necessary for cAMP maximal induction. To study the molecular events mediating the cAMP induction, DNase I footprinting and electromobility shift assays (EMSAs) were performed with renin-producing chorionic cell and kidney cortex cell nuclear extracts, showing that the CRE-binding protein (CREB) interacts with the CRE and that tissue-specific factors, distinct from Pit-1, specifically bind the renin Pit-1 motif. Taken together, these results demonstrate that the cAMP response of the human renin gene may involve CREB binding the CRE and tissue-specific factors, different from Pit-1, that interact with the Pit-1 response DNA elements.
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