ArticleThe Biochemical journal1996
Oxytocin receptor couples to the 80 kDa Gh alpha family protein in human myometrium.
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 52 citations in OpenAlex.
- Oxytocin receptor single nucleotide polymorphism predicts atony-related postpartum hemorrhage.BMC pregnancy and childbirth · 2022Article
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- Computational physiology of uterine smooth muscle.Science progress · 2019Article
- Article
- Opening up about Tissue Transglutaminase: When Conformation Matters More than Enzymatic Activity.Med one · 2018Article
- Induction of GD3/α1-adrenergic receptor/transglutaminase 2-mediated erythroid differentiation in chronic myelogenous leukemic K562 cells.Oncotarget · 2017Article
- The Gαh-PLCδ1 signaling axis drives metastatic progression in triple-negative breast cancer.Journal of hematology & oncology · 2017Article
- Physiological, pathological, and structural implications of non-enzymatic protein-protein interactions of the multifunctional human transglutaminase 2.Cellular and molecular life sciences : CMLS · 2015Review
- Tissue transglutaminase contributes to the pathogenesis of preeclampsia and stabilizes placental angiotensin receptor type 1 by ubiquitination-preventing isopeptide modification.Hypertension (Dallas, Tex. : 1979) · 2014Article
- Biological functionalities of transglutaminase 2 and the possibility of its compensation by other members of the transglutaminase family.TheScientificWorldJournal · 2014Review
- The essential role of Giα2 in prostate cancer cell migration.Molecular cancer research : MCR · 2012Article
- Transglutaminase 2: a molecular Swiss army knife.Biochimica et biophysica acta · 2012Review
- Oxytocin induces the migration of prostate cancer cells: involvement of the Gi-coupled signaling pathway.Molecular cancer research : MCR · 2010Article
- Roles of transglutaminases in cardiac and vascular diseases.Frontiers in bioscience : a journal and virtual library · 2007Review
- Novel bimodal effects of the G-protein tissue transglutaminase on adrenoreceptor signalling.The Biochemical journal · 1999Article
- Interactions of G(h)/transglutaminase with phospholipase Cdelta1 and with GTP.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Phospholipase C-delta1 and oxytocin receptor signalling: evidence of its role as an effector.The Biochemical journal · 1998Article
- Molecular mechanisms of parturition.Infectious diseases in obstetrics and gynecology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the primary functions of the oxytocin receptor is to modulate intracellular calcium levels in myometrium. The oxytocin receptor has been purified and cloned. Although it has been suggested that oxytocin receptor couples with a GTP-binding regulatory protein (G-protein), the identity of this G-protein remains unclear. To elucidate the mechanism of oxytocin receptor signalling, we used the oxytocin-receptor-G-protein ternary complex preparation from human myometrium, and evaluated oxytocin-mediated activation of [35S]guanosine 5'-[gamma-thio]triphosphate ([35S]GTP[S]) binding and [alpha-32P]GTP photoaffinity labelling to a G-protein. Binding of [35S]GTP[S] and the intensity of the [alpha-32P]GTP photoaffinity labelled protein resulting from activation of the oxytocin receptor were significantly attenuated by the selective oxytocin antagonist, desGlyNH2d(CH2)5[Tyr(Me)2,Thr4]OVT. Furthermore, the molecular mass of the specific GTP-binding protein was approximately 80 kDa; homologous with the Gh alpha family, the new class of GTP-binding proteins first identified in rat liver that couples to the alpha 1B-adrenoceptor. Consistent with these observations, in co-immunoprecipitation and co-immunoadsorption of the oxytocin receptor in the ternary complex preparation by anti-Gh7 alpha antibody, the Gh alpha family protein tightly coupled to the oxytocin receptor. These findings demonstrate that oxytocin receptor couples with approximately 80 kDa Gh alpha in signal mediation.
Indexed as
Identifiers
8645152PMC1217269W1748943496What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.