ArticleThe Biochemical journal1996
Purification and characterization of a recombinant human Theta-class glutathione transferase (GSTT2-2).
Article in The Biochemical journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Proanthocyanidins mitigate bile acid-induced changes in GSTT2 levels in a panel of racially diverse patient-derived primary esophageal cell cultures.Molecular carcinogenesis · 2022Article
- Copy Number Variation of Circulating Tumor DNA (ctDNA) Detected Using NIPT in Neoadjuvant Chemotherapy-Treated Ovarian Cancer Patients.Frontiers in genetics · 2022Article
- Article
- Dietary iso-α-acids prevent acetaldehyde-induced liver injury through Nrf2-mediated gene expression.PloS one · 2021Article
- Infection by a Giant Virus (AaV) Induces Widespread Physiological Reprogramming inFrontiers in microbiology · 2018Article
- Determination of oxidative stress and activities of antioxidant enzymes in guinea pigs treated with haloperidol.Experimental and therapeutic medicine · 2013Article
- Article
- Gene expression profiles in human peripheral blood mononuclear cells as biomarkers for nutritional in vitro and in vivo investigations.Genes & nutrition · 2010Article
- Glutathione transferases.The arabidopsis book · 2010Article
- Linkage disequilibrium between two high-frequency deletion polymorphisms: implications for association studies involving the glutathione-S transferase (GST) genes.PLoS genetics · 2009Article
- Intervention with cloudy apple juice results in altered biological activities of ileostomy samples collected from individual volunteers.European journal of nutrition · 2008Article
- GSTT2 promoter polymorphisms and colorectal cancer risk.BMC cancer · 2007Article
- Evolution of highly active enzymes by homology-independent recombination.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Ure2, a prion precursor with homology to glutathione S-transferase, protects Saccharomyces cerevisiae cells from heavy metal ion and oxidant toxicity.The Journal of biological chemistry · 2003Article
- Identification and characterization of GSTT3, a third murine Theta class glutathione transferase.The Biochemical journal · 2002Article
- Expression of glutathione S-transferase alpha, P1-1 and T1-1 in the human gastrointestinal tract.Japanese journal of cancer research : Gann · 2000Article
- Mutagenic analysis of conserved arginine residues in and around the novel sulfate binding pocket of the human Theta class glutathione transferase T2-2.Protein science : a publication of the Protein Society · 1999Article
- Gene structure, expression and chromosomal localization of murine theta class glutathione transferase mGSTT1-1.The Biochemical journal · 1999Article
- Identification of cDNAs encoding two human alpha class glutathione transferases (GSTA3 and GSTA4) and the heterologous expression of GSTA4-4.The Biochemical journal · 1998Article
- Structure and expression of a cluster of glutathione S-transferase genes from a marine fish, the plaice (Pleuronectes platessa).The Biochemical journal · 1997Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding the human Theta-class glutathione transferase GSTT2-2 was expressed in Escherichia coli as a ubiquitin fusion protein. The co-translational removal of the ubiquitin by a cloned ubiquitin-specific protease, Ubp1, generates enzymically active GSTT2-2 without any additional N-terminal residues. The recombinant isoenzyme was purified to apparent homogeneity by DEAE anion-exchange, gel filtration, dye ligand chromatography and high resolution anion-exchange chromatography on Mono Q FPLC. The recombinant enzyme had significant activity with a range of substrates, including cumene hydroperoxide and 1-menapthyl sulphate. The activity of GSTT2-2 with a range of secondary lipid peroxidation products such as the trans,trans-alka-2,4-dienals and trans-alk-2-enals, as well as its glutathione peroxidase activity with organic hydroperoxides, suggest that it may play a significant role in protection against the products of lipid peroxidation.
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