ArticleAmerican journal of human genetics1996
Assessment of aneuploidy for chromosomes 8, 9, 13, 16, and 21 in human sperm by using primed in situ labeling technique.
Article in American journal of human genetics, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 54 citations in OpenAlex.
- Preliminary analysis of numerical chromosome abnormalities in reciprocal and Robertsonian translocation preimplantation genetic diagnosis cases with 24-chromosomal analysis with an aCGH/SNP microarray.Journal of assisted reproduction and genetics · 2018Article
- Efficient combined FISH and PRINS technique for detection of DAZ microdeletion in human sperm.Journal of assisted reproduction and genetics · 2012Article
- Impact of DNA damage on the frequency of sperm chromosomal aneuploidy in normal and subfertile men.Iranian biomedical journal · 2011Article
- The peptide nucleic acids (PNAs): introduction to a new class of probes for chromosomal investigation.Chromosoma · 2004Review
- Ultra-rapid multicolor PRINS protocol for chromosome detection in human sperm.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2002Article
- Strategies for signal amplification in nucleic acid detection.Molecular biotechnology · 2001Review
- Fluorescence in situ hybridization with chromosome paint probes: a novel approach to assess aneuploidy in human sperm nuclei.Journal of assisted reproduction and genetics · 1999Article
- Rapid chromosome detection in human gametes, zygotes, and preimplantation embryos using the PRINS technique.Journal of assisted reproduction and genetics · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The incidence of aneuploidy was estimated for chromosomes 8, 9, 13, 16, and 21 in mature human spermatozoa by primed in situ (PRINS) labeling technique. This method allows us to perform a chromosome-specific detection by in situ annealing of a centromeric specific primer. A dual color PRINS protocol was adapted to human sperm. The decondensation and the denaturation of sperm nuclei were simultaneously performed by 3-M NaOH treatment. Double labeling of spermatozoa was obtained in <2 h. A total of 96,292 sperm nuclei were analyzed by two independent observers. The estimates of disomy were 0.31% for chromosome 8, 0.28% for chromosome 9, 0.28% for chromosome 13, 0.26% for chromosome 16, and 0.32% for chromosome 21. These homogeneous findings suggest an equal distribution of aneuploidies among autosomal chromosomes in males.
Indexed as
Identifiers
8644744PMC1914680W1563989990What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.