ArticleProceedings of the National Academy of Sciences of the United States of America1996
An induction gene trap screen in embryonic stem cells: Identification of genes that respond to retinoic acid in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Modeling the study of DNA damage responses in mice.Methods in molecular biology (Clifton, N.J.) · 2015Review
- Experimental validation of Ankrd17 and Anapc10, two novel meiotic genes predicted by computational models in mice.Biology of reproduction · 2012Article
- The mouse genetics toolkit: revealing function and mechanism.Genome biology · 2011Review
- Development of the circadian oscillator during differentiation of mouse embryonic stem cells in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Gene trap mutagenesis in the mouse.Methods in enzymology · 2010Article
- Gene trap mutagenesis in mice: new perspectives and tools in cancer research.Cancer science · 2008Review
- Cre recombinase mediated alterations of the mouse genome using embryonic stem cells.Methods in molecular biology (Clifton, N.J.) · 2008Review
- Identifying genes preferentially expressed in undifferentiated embryonic stem cells.BMC cell biology · 2007Article
- Insertional mutagenesis strategies in zebrafish.Genome biology · 2007Review
- Inducible gene trapping with drug-selectable markers and Cre/loxP to identify developmentally regulated genes.Molecular and cellular biology · 2004Article
- Identification of Jade1, a gene encoding a PHD zinc finger protein, in a gene trap mutagenesis screen for genes involved in anteroposterior axis development.Molecular and cellular biology · 2003Article
- Gene trap mutagenesis of hnRNP A2/B1: a cryptic 3' splice site in the neomycin resistance gene allows continued expression of the disrupted cellular gene.BMC genomics · 2003Article
- Gene trap vector screen for developmental genes in differentiating ES cells.Methods in enzymology · 2003Article
- Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Genomewide trapping of genes that encode secreted and transmembrane proteins repressed by oncogenic signaling.Genome research · 2001Article
- Functional genomics of the murine immune system.Immunologic research · 2001Review
- Identification of an enhancer and an alternative promoter in the first intron of the alpha-fetoprotein gene.Nucleic acids research · 2000Article
- E-MAP-115, encoding a microtubule-associated protein, is a retinoic acid-inducible gene required for spermatogenesis.Genes & development · 2000Article
- Identification of a series of transforming growth factor beta-responsive genes by retrovirus-mediated gene trap screening.Molecular and cellular biology · 2000Article
- Embryonic stem cell differentiation models: cardiogenesis, myogenesis, neurogenesis, epithelial and vascular smooth muscle cell differentiation in vitro.Cytotechnology · 1999Article
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8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a novel induction gene trap approach that preselects in vitro for integrations into genes that lie downstream of receptor/ligand-mediated signaling pathways. Using this approach, we have identified 20 gene trap integrations in embryonic stem cells, 9 of which were induced and 11 of which were repressed after exposure to exogenous retinoic acid (RA). All but one of these integrations showed unique spatially restricted or tissue-specific patterns of expression between 8.5 and 11.5 days of embryogenesis. Interestingly, expression was observed in tissues that are affected by alterations in RA levels during embryogenesis. Sequence analysis of fusion transcripts from six integrations revealed five novel gene sequences and the previously identified protooncogene c-fyn. To date, germ-line transmission and breeding has uncovered one homozygous embryonic lethal and three homozygous viable insertions. These studies demonstrate the potential of this induction gene trap approach for identifying and mutating genes downstream of signal transduction pathways.
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