ArticleProceedings of the National Academy of Sciences of the United States of America1996
cdc18+ regulates initiation of DNA replication in Schizosaccharomyces pombe.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 126 citations in OpenAlex.
- DNA Replication Determines Timing of Mitosis by Restricting CDK1 and PLK1 Activation.Molecular cell · 2018Article
- The Protein Level of Rev1, a TLS Polymerase in Fission Yeast, Is Strictly Regulated during the Cell Cycle and after DNA Damage.PloS one · 2015Article
- Regulating DNA replication in eukarya.Cold Spring Harbor perspectives in biology · 2013Review
- Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library.BMC genomics · 2012Article
- Plant geminivirus rep protein induces rereplication in fission yeast.Journal of virology · 2009Article
- Checkpoint effects and telomere amplification during DNA re-replication in fission yeast.BMC molecular biology · 2007Article
- Cdc6 ATPase activity regulates ORC x Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication.The Journal of biological chemistry · 2007Article
- The CENP-B homolog, Abp1, interacts with the initiation protein Cdc23 (MCM10) and is required for efficient DNA replication in fission yeast.Cell division · 2006Article
- UV irradiation induces a postreplication DNA damage checkpoint.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Production of reactive oxygen species in response to replication stress and inappropriate mitosis in fission yeast.Journal of cell science · 2006Article
- Disruption of mechanisms that prevent rereplication triggers a DNA damage response.Molecular and cellular biology · 2005Article
- Overexpression of the replication licensing regulators hCdt1 and hCdc6 characterizes a subset of non-small-cell lung carcinomas: synergistic effect with mutant p53 on tumor growth and chromosomal instability--evidence of E2F-1 transcriptional control over hCdt1.The American journal of pathology · 2004Article
- Interaction of the S-phase cyclin Clb5 with an "RXL" docking sequence in the initiator protein Orc6 provides an origin-localized replication control switch.Genes & development · 2004Article
- Eukaryotic MCM proteins: beyond replication initiation.Microbiology and molecular biology reviews : MMBR · 2004Review
- Site-specific ORC binding, pre-replication complex assembly and DNA synthesis at Schizosaccharomyces pombe replication origins.The EMBO journal · 2002Article
- Redundant control of rereplication in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Chromatin association of human origin recognition complex, cdc6, and minichromosome maintenance proteins during the cell cycle: assembly of prereplication complexes in late mitosis.Molecular and cellular biology · 2000Article
- Cdc6p modulates the structure and DNA binding activity of the origin recognition complex in vitro.Genes & development · 2000Article
- Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18.The EMBO journal · 2000Article
- PR48, a novel regulatory subunit of protein phosphatase 2A, interacts with Cdc6 and modulates DNA replication in human cells.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the fission yeast Schizosaccharomyces pombe the cdc18'+gene is required both for initiation of DNA replication and for coupling mitosis to the completion of S phase. Cells lacking Cdc18 fail to enter S phase but still undergo nuclear division. Expression of cdc18+ is sufficient to drive a G1-arrested cdc10ts mutant into the S phase of the cell cycle, indicating that cdc18+ represents a critical link between passage through START and the initiation of DNA replication. Here we show that Cdcl8 is a highly unstable protein that is expressed only once per cell cycle at the boundary between GI and S phase. De novo synthesis of Cdc18 is required before, but not after, the initiation of DNA replication, indicating that Cdc18 function is not necessary once the initiation event has occurred. Overproduction of the protein results in an accumulation of cells with DNA content of greater than 2C and delays mitosis, suggesting that Cdc18 is sufficient to cause reinitiation of DNA replication within a given cell cycle. Our data indicate that the synthesis of Cdc18 protein is a critical rate-limiting step in the initiation of DNA replication during each cell cycle. The extreme lability of the protein may contribute to the prevention of reinitiation.
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