ArticleProceedings of the National Academy of Sciences of the United States of America1996
Differentiation of the immortalized adult neuronal progenitor cell line HC2S2 into neurons by regulatable suppression of the v-myc oncogene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 163 citations in OpenAlex.
- Review
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- Stretch-induced actin remodeling requires targeting of zyxin to stress fibers and recruitment of actin regulators.Molecular biology of the cell · 2012Article
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- TRIF modulates TLR5-dependent responses by inducing proteolytic degradation of TLR5.The Journal of biological chemistry · 2010Article
- The LIM Protein Zyxin Binds CARP-1 and Promotes Apoptosis.Genes & cancer · 2010Article
- Myc down-regulation affects cyclin D1/cdk4 activity and induces apoptosis via Smac/Diablo pathway in an astrocytoma cell line.Cell proliferation · 2009Article
- Regional differences in the temporal expression of non-apoptotic caspase-3-positive bergmann glial cells in the developing rat cerebellum.Frontiers in neuroanatomy · 2009Article
- Genetic ablation of zyxin causes Mena/VASP mislocalization, increased motility, and deficits in actin remodeling.The Journal of cell biology · 2006Article
- Mechanical force mobilizes zyxin from focal adhesions to actin filaments and regulates cytoskeletal reinforcement.The Journal of cell biology · 2005Article
- Overexpression of c-MYC promotes an undifferentiated phenotype in cultured astrocytes and allows elevated Ras and Akt signaling to induce gliomas from GFAP-expressing cells in mice.Neuron glia biology · 2004Article
- Estradiol differentially regulates c-Fos after focal cerebral ischemia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- HMG box transcription factor TCF-4's interaction with CtBP1 controls the expression of the Wnt target Axin2/Conductin in human embryonic kidney cells.Nucleic acids research · 2003Article
- Mammalian neural stem-cell renewal: nature versus nurture.Molecular neurobiology · 2003Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
A regulatable retroviral vector in which the v-myc oncogene is driven by a tetracycline-controlled transactivator and a human cytomegalovirus minimal promoter fused to a tet operator sequence was used for conditional immortalization of adult rat neuronal progenitor cells. A single clone, HC2S2, was isolated and characterized. Two days after the addition of tetracycline, the HC2S2 cells stopped proliferating, began to extend neurites, and expressed the neuronal markers tau, NeuN, neurofilament 200 kDa, and glutamic acid decarboxylase in accordance with the reduced production of the v-myc oncoprotein. Differentiated HC2S2 cells expressed large sodium and calcium currents and could fire regenerative action potentials. These results suggest that the suppression of the v-myc oncogene may be sufficient to make proliferating cells exit from cell cycles and induce terminal differentiation. The HC2S2 cells will be valuable for studying the differentiation process of neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.