ArticleProceedings of the National Academy of Sciences of the United States of America1996
In vivo protein-DNA interactions at human DNA replication origin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- Multi-step loading of human minichromosome maintenance proteins in live human cells.The Journal of biological chemistry · 2013Article
- Origin of DNA replication at the human lamin B2 locus: OBR or ABR?Cell cycle (Georgetown, Tex.) · 2012Article
- Back to the origin: reconsidering replication, transcription, epigenetics, and cell cycle control.Genes & cancer · 2012Article
- The epigenetic control of E-box and Myc-dependent chromatin modifications regulate the licensing of lamin B2 origin during cell cycle.Nucleic acids research · 2012Article
- Differential chromatin structure encompassing replication origins in transformed and normal cells.Genes & cancer · 2012Article
- Homeotic proteins participate in the function of human-DNA replication origins.Nucleic acids research · 2010Article
- Differences in the DNA replication of unicellular eukaryotes and metazoans: known unknowns.EMBO reports · 2010Review
- Genomic study of replication initiation in human chromosomes reveals the influence of transcription regulation and chromatin structure on origin selection.Molecular biology of the cell · 2010Article
- Functional interactions of DNA topoisomerases with a human replication origin.The EMBO journal · 2007Article
- Defined sequence modules and an architectural element cooperate to promote initiation at an ectopic mammalian chromosomal replication origin.Molecular and cellular biology · 2004Article
- Modular structure of the human lamin B2 replicator.Molecular and cellular biology · 2004Article
- Localization of proteins bound to a replication origin of human DNA along the cell cycle.The EMBO journal · 2003Article
- A approximately 35 kDa polypeptide from insect cells binds to yeast ACS like elements in the presence of ATP.BMC biochemistry · 2002Article
- The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.Molecular and cellular biology · 2001Article
- Recruitment of a basal polyadenylation factor by the upstream sequence element of the human lamin B2 polyadenylation signal.Molecular and cellular biology · 2000Article
- Attachment to the nuclear matrix mediates specific alterations in chromatin structure.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The Bacteroides fragilis BtgA mobilization protein binds to the oriT region of pBFTM10.Journal of bacteriology · 1998Article
- Cell cycle modulation of protein-DNA interactions at a human replication origin.The EMBO journal · 1998Article
- Initiation of DNA replication at CpG islands in mammalian chromosomes.The EMBO journal · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-DNA interactions were studied in vivo at the region containing a human DNA replication origin, located at the 3' end of the lamin B2 gene and partially overlapping the promoter of another gene, located downstream. DNase I treatment of nuclei isolated from both exponentially growing and nonproliferating HL-60 cells showed that this region has an altered, highly accessible, chromatin structure. High-resolution analysis of protein-DNA interactions in a 600-bp area encompassing the origin was carried out by the in vivo footprinting technique based on the ligation-mediated polymerase chain reaction. In growing HL-60 cells, footprints at sequences homologous to binding sites for known transcription factors (members of the basic-helix-loop-helix family, nuclear respiratory factor 1, transcription factor Sp1, and upstream binding factor) were detected in the region corresponding to the promoter of the downstream gene. Upon conversion of cells to a nonproliferative state, a reduction in the intensity of these footprints was observed that paralleled the diminished transcriptional activity of the genomic area. In addition to these protections, in close correspondence to the replication initiation site, a prominent footprint was detected that extended over 70 nucleotides on one strand only. This footprint was absent from nonproliferating HL-60 cells, indicating that this specific protein-DNA interaction might be involved in the process of origin activation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.