ArticleProceedings of the National Academy of Sciences of the United States of America1996
The human lysozyme promoter directs reporter gene expression to activated myelomonocytic cells in transgenic mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 37 citations in OpenAlex.
- Macrophage deletion of Noc4l triggers endosomal TLR4/TRIF signal and leads to insulin resistance.Nature communications · 2021Article
- Direct conversion of injury-site myeloid cells to fibroblast-like cells of granulation tissue.Nature communications · 2018Article
- Imaging of Tumor-Associated Macrophages in a Transgenic Mouse Model of Orthotopic Ovarian Cancer.Molecular imaging and biology · 2017Article
- The p38alpha mitogen-activated protein kinase limits the CNS proinflammatory cytokine response to systemic lipopolysaccharide, potentially through an IL-10 dependent mechanism.Journal of neuroinflammation · 2014Article
- The p38α MAPK regulates microglial responsiveness to diffuse traumatic brain injury.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Identification of an intestine-specific promoter and inducible expression of bacterial α-galactosidase in mammalian cells by a lac operon system.Journal of animal science and biotechnology · 2012Article
- Microglial p38α MAPK is critical for LPS-induced neuron degeneration, through a mechanism involving TNFα.Molecular neurodegeneration · 2011Article
- Inducible transgenes under the control of the hCD68 promoter identifies mouse macrophages with a distribution that differs from the F4/80 - and CSF-1R-expressing populations.Experimental hematology · 2009Article
- Article
- Efficacy of adenoviral TNF alpha antisense is enhanced by a macrophage specific promoter.Gene therapy · 2001Article
- Conditional gene targeting in macrophages and granulocytes using LysMcre mice.Transgenic research · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 5' region of the human lysozyme gene from -3500 to +25 was fused to a chloramphenicol acetyltransferase (CAT) reporter gene and three transgenic founder mice were obtained. All three transgenic lines showed the same pattern of CAT enzyme expression in adult mouse tissues that was consistent with the targeting of elicited, activated macrophages in tissues and developing and elicited granulocytes. In normal mice high CAT enzyme activity was found in the spleen, lung, and thymus, tissues rich in phagocytically active cells, but not in many other tissues, such as the gut and muscle, which contain resident macrophages. Cultured resident peritoneal macrophages and cells elicited 18 hr (granulocytes) and 4 days (macrophages) after injection of sterile thioglycollate broth expressed CAT activity. Bacillus Calmette-Guérin infection of transgenic mice resulted in CAT enzyme expression in the liver, which contained macrophage-rich granulomas, whereas the liver of uninfected mice did not have any detectable CAT enzyme activity. Although the Paneth cells of the small intestine in both human and mouse produce lysozyme, the CAT gene, under the control of the human lysozyme promoter, was not expressed in the mouse small intestine. These results indicate that the human lysozyme promoter region may be used to direct expression of genes to activated mouse myeloid cells.
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