ArticleProceedings of the National Academy of Sciences of the United States of America1996
Localization of 17beta-hydroxysteroid dehydrogenase and characterization of testosterone in the brain of the male frog.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 115 citations in OpenAlex.
- The Role of Sex and Sex Hormones in Neurodegenerative Diseases.Endocrine reviews · 2020Review
- Allopregnanolone and Progesterone in Experimental Neuropathic Pain: Former and New Insights with a Translational Perspective.Cellular and molecular neurobiology · 2019Review
- The non-benzodiazepine anxiolytic drug etifoxine causes a rapid, receptor-independent stimulation of neurosteroid biosynthesis.PloS one · 2015Article
- Biosynthesis and biological action of pineal allopregnanolone.Frontiers in cellular neuroscience · 2014Review
- The implication of neuroactive steroids in Tourette's syndrome pathogenesis: A role for 5α-reductase?Journal of neuroendocrinology · 2013Review
- New biosynthesis and biological actions of avian neurosteroids.Journal of experimental neuroscience · 2013Review
- Regulation of neurosteroid biosynthesis by neurotransmitters and neuropeptides.Frontiers in endocrinology · 2012Article
- Mode of action and functional significance of 7α-hydroxypregnenolone stimulating locomotor activity.Frontiers in endocrinology · 2011Article
- Neurosteroid biosynthesis and function in the brain of domestic birds.Frontiers in endocrinology · 2011Article
- Neurosteroid biosynthesis in the brain of amphibians.Frontiers in endocrinology · 2011Article
- Measurement of steroid concentrations in brain tissue: methodological considerations.Frontiers in endocrinology · 2011Article
- 7α-hydroxypregnenolone, a new key regulator of locomotor activity of vertebrates: identification, mode of action, and functional significance.Frontiers in endocrinology · 2010Article
- Direct action of gonadotropin in brain integrates behavioral and reproductive functions.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Vasotocin and mesotocin stimulate the biosynthesis of neurosteroids in the frog brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2006Article
- Neurogenic pain and steroid synthesis in the spinal cord.Journal of molecular neuroscience : MN · 2006Review
- Biosynthesis and organizing action of neurosteroids in the developing Purkinje cell.Cerebellum (London, England) · 2006Review
- Substance P inhibits progesterone conversion to neuroactive metabolites in spinal sensory circuit: a potential component of nociception.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- 7alpha-Hydroxypregnenolone acts as a neuronal activator to stimulate locomotor activity of breeding newts by means of the dopaminergic system.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- A novel aspect of the cerebellum: biosynthesis of neurosteroids in the Purkinje cell.Cerebellum (London, England) · 2003Review
- Oestrogen regulates male aggression in the non-breeding season.Proceedings. Biological sciences · 2000Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several enzymes involved in the formation of steroids of the pregnene and pregnane series have been identified in the brain, but the biosynthesis of testosterone has never been reported in the central nervous system. In the present study, we have investigated the distribution and bioactivity of 17beta-hydroxysteroid dehydrogenase (17beta-HSD) (EC 1.1.1.62; a key enzyme that is required for the formation of testosterone and estradiol) in the brain of the male frog Rana ridibunda. By using an antiserum against human type I placental 17beta-HSD, immunoreactivity was localized in a discrete group of ependymal glial cells bordering the telencephalic ventricles. HPLC analysis of telencephalon and hypothalamus extracts combined with testosterone radioimmunoassay revealed the existence of two peaks coeluting with testosterone and 5alpha-dihydrotestosterone. After HPLC purification, testosterone was identified by gas chromatography/mass spectrometry. Incubation of telencephalon slices with [3H]pregnenolone resulted in the formation of metabolites which coeluted with progesterone, 17alpha-hydroxyprogesterone, dehydroepiandrosterone, androstenedione, testosterone, and 5alpha-dihydrotestosterone. The newly synthesized steroid comigrating with testosterone was selectively immunodetected by using testosterone antibodies. These data indicate that 17beta-HSD is expressed in a subpopulation of gliocytes in the frog telencephalon and that telencephalic cells are capable of synthesizing various androgens, including dehydroepiandrosterone, androstenedione, testosterone, and 5alpha-dihydrotestosterone.
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