ArticleProceedings of the National Academy of Sciences of the United States of America1996
Near-membrane [Ca2+] transients resolved using the Ca2+ indicator FFP18.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 92 citations in OpenAlex.
- The Different Facets of Extracellular Calcium Sensors: Old and New Concepts in Calcium-Sensing Receptor Signalling and Pharmacology.International journal of molecular sciences · 2018Review
- Recent advances in understanding the extracellular calcium-sensing receptor.F1000Research · 2016Review
- Novel features on the regulation by mitochondria of calcium and secretion transients in chromaffin cells challenged with acetylcholine at 37°C.Physiological reports · 2013Article
- Pericellular Ca(2+) recycling potentiates thrombin-evoked Ca(2+) signals in human platelets.Physiological reports · 2013Article
- Subplasma membrane Ca2+ signals.IUBMB life · 2012Review
- A toolset of aequorin expression vectors for in planta studies of subcellular calcium concentrations in Arabidopsis thaliana.Journal of experimental botany · 2012Article
- Activity-dependent regulation of the binomial parameters p and n at the mouse neuromuscular junction in vivo.Journal of neurophysiology · 2010Article
- Chemical calcium indicators.Methods (San Diego, Calif.) · 2008Review
- A close association of RyRs with highly dense clusters of Ca2+-activated Cl- channels underlies the activation of STICs by Ca2+ sparks in mouse airway smooth muscle.The Journal of general physiology · 2008Article
- Regulation of smooth muscle excitation and contraction.Neurogastroenterology and motility · 2008Review
- Article
- Biphasic Ca2+-dependent switching in a calmodulin-IQ domain complex.Biochemistry · 2006Article
- Visualization of localized store-operated calcium entry in mouse astrocytes. Close proximity to the endoplasmic reticulum.The Journal of physiology · 2005Article
- Regulation of basal intracellular calcium concentration by the sarcoplasmic reticulum in myocytes from the rat gastric antrum.The Journal of physiology · 2000Article
- Chimeric green fluorescent protein-aequorin as bioluminescent Ca2+ reporters at the single-cell level.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Elevated subsarcolemmal Ca2+ in mdx mouse skeletal muscle fibers detected with Ca2+-activated K+ channels.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Multiple pathways responsible for the stretch-induced increase in Ca2+ concentration in toad stomach smooth muscle cells.The Journal of physiology · 2000Article
- Carbachol-induced [Ca2+]i increase, but not activation of protein kinase C, stimulates exocytosis in rat parotid acini.The Journal of physiology · 2000Article
- Kinetics of contraction in depolarized smooth muscle from guinea-pig taenia coli after photodestruction of nifedipine.The Journal of physiology · 1999Article
- Distribution of active protein kinase C in smooth muscle.Biophysical journal · 1999Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
(Ca2+)-sensitive processes at cell membranes involved in contraction, secretion, and neurotransmitter release are activated in situ or in vitro by Ca2+ concentrations ([Ca2+]) 10-100 times higher than [Ca2+] measured during stimulation in intact cells. This paradox might be explained if the local [Ca2+] at the cell membrane is very different from that in the rest of the cell. Soluble Ca2+ indicators, which indicate spatially averaged cytoplasmic [Ca2+], cannot resolve these localized, near-membrane [Ca2+] signals. FFP18, the newest Ca2+ indicator designed to selectively monitor near-membrane [Ca2+], has a lower Ca2+ affinity and is more water soluble than previously used membrane-associating Ca2+ indicators. Images of the intracellular distribution of FFP18 show that >65% is located on or near the plasma membrane. [Ca2+] transients recorded using FFP18 during membrane depolarization-induced Ca2+ influx show that near-membrane [Ca2+] rises faster and reaches micromolar levels at early times when the cytoplasmic [Ca2+], recorded using fura-2, has risen to only a few hundred nanomolar. High-speed series of digital images of [Ca2+] show that near-membrane [Ca2+], reported by FFP18, rises within 20 msec, peaks at 50-100 msec, and then declines. [Ca2+] reported by fura-2 rose slowly and continuously throughout the time images were acquired. The existence of these large, rapid increases in [Ca2+] directly beneath the surface membrane may explain how numerous (Ca2+)-sensitive membrane processes are activated at times when bulk cytoplasmic [Ca2+] changes are too small to activate them.
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