ArticleProceedings of the National Academy of Sciences of the United States of America1996
Functional expression of the Schizosaccharomyces pombe Na+/H+ antiporter gene, sod2, in Saccharomyces cerevisiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 59 citations in OpenAlex.
- Characterization of Critical Amino Acids in the Transport and Selectivity of the Plant NaInternational journal of molecular sciences · 2025Article
- Up-regulation of plasma membrane HMolecular biology research communications · 2014Article
- Paradigm for industrial strain improvement identifies sodium acetate tolerance loci in Zymomonas mobilis and Saccharomyces cerevisiae.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Electrical control of cell polarization in the fission yeast Schizosaccharomyces pombe.Current biology : CB · 2010Article
- Alkali metal cation transport and homeostasis in yeasts.Microbiology and molecular biology reviews : MMBR · 2010Review
- Growth at high pH and sodium and potassium tolerance in media above the cytoplasmic pH depend on ENA ATPases in Ustilago maydis.Eukaryotic cell · 2009Article
- Plant NHX cation/proton antiporters.Plant signaling & behavior · 2009Review
- Exclusion of Na+ via sodium ATPase (PpENA1) ensures normal growth of Physcomitrella patens under moderate salt stress.Plant physiology · 2007Article
- Overexpression of SOD2 increases salt tolerance of Arabidopsis.Plant physiology · 2003Article
- Functional analysis of amino acids of the Na+/H+ exchanger that are important for proton translocation.Molecular and cellular biochemistry · 2003Article
- The putative plasma membrane Na(+)/H(+) antiporter SOS1 controls long-distance Na(+) transport in plants.The Plant cell · 2002Article
- Cloning and expression of two genes coding for sodium pumps in the salt-tolerant yeast Debaryomyces hansenii.Journal of bacteriology · 2001Article
- Na+/H+ antiporter from Synechocystis species PCC 6803, homologous to SOS1, contains an aspartic residue and long C-terminal tail important for the carrier activity.Plant physiology · 2001Article
- The Arabidopsis thaliana salt tolerance gene SOS1 encodes a putative Na+/H+ antiporter.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Altered Na+ and Li+ homeostasis in Saccharomyces cerevisiae cells expressing the bacterial cation antiporter NhaA.Journal of bacteriology · 1998Article
- The transcriptional activator Imp2p maintains ion homeostasis in Saccharomyces cerevisiae.Genetics · 1998Article
- Postantibiotic effect in Escherichia coli determined with real-time metabolic monitoring.Antimicrobial agents and chemotherapy · 1998Article
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3 authors at 1 institution.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the fission yeast, Schizosaccharomyces pombe, tolerance to high sodium and lithium concentrations requires the functioning of the sod2, Na+/H+ antiporter. We have directly measured the activity of this antiporter and demonstrated reconstitution of the activity in gene deletion strains. In addition, we have shown that it can be transferred to, and its antiporter activity detected in, the budding yeast, Saccharomyces cerevisiae, where it also confers sodium and lithium tolerance. Proton flux through the S. pombe Na+/H+ antiporter was directly measured using microphysiometry. The direction of transmembrane proton flux mediated by this antiporter was reversible, with protons being imported or exported in response to the external concentration of sodium. This bidirectional activity was also detected in S. cerevisiae strains expressing sod2 and expression of this gene complemented the sodium and lithium sensitivity resulting from inactivation of the ENA1/PMR2 encoded Na+-exporting ATPases. This suggests that antiporters or sodium pumps can be utilized interchangeably by S. cerevisiae to regulate internal sodium concentration. Potent inhibitors of mammalian Na+/H+ exchangers were found to have no effect on sod2 activity. The proton flux mediated by sod2 was also found to be unaffected by perturbation of membrane potential or the plasma membrane proton gradient.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.