ArticleProceedings of the National Academy of Sciences of the United States of America1996
An AML-1 consensus sequence binds an osteoblast-specific complex and transcriptionally activates the osteocalcin gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 169 citations in OpenAlex.
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- Osteogenic differentiation characteristics of hip joint capsule fibroblasts obtained from patients with ankylosing spondylitis.Annals of translational medicine · 2021Article
- Mll-COMPASS complexes mediate H3K4me3 enrichment and transcription of the osteoblast master gene Runx2/p57 in osteoblasts.Journal of cellular physiology · 2019Article
- Bushen Qiangji Granule () medicated serum inhibits osteogenic differentiation of fibroblasts in ankylosing spondylitis by inhibiting the BMP/Smads signal pathway in vitro.Chinese journal of integrative medicine · 2016Article
- Polycomb PRC2 complex mediates epigenetic silencing of a critical osteogenic master regulator in the hippocampus.Biochimica et biophysica acta · 2016Article
- Bushen-Qiangdu-Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43.Experimental and therapeutic medicine · 2016Article
- Epigenetic Control of the Bone-master Runx2 Gene during Osteoblast-lineage Commitment by the Histone Demethylase JARID1B/KDM5B.The Journal of biological chemistry · 2015Article
- Article
- Non-viral delivery of inductive and suppressive genes to adipose-derived stem cells for osteogenic differentiation.Pharmaceutical research · 2011Article
- Foxo1 mediates insulin-like growth factor 1 (IGF1)/insulin regulation of osteocalcin expression by antagonizing Runx2 in osteoblasts.The Journal of biological chemistry · 2011Article
- The Role of RUNX2 in Osteosarcoma Oncogenesis.Sarcoma · 2011Article
- Critical role of activating transcription factor 4 in the anabolic actions of parathyroid hormone in bone.PloS one · 2009Article
- Parathyroid hormone increases activating transcription factor 4 expression and activity in osteoblasts: requirement for osteocalcin gene expression.Endocrinology · 2008Article
- General transcription factor IIA-gamma increases osteoblast-specific osteocalcin gene expression via activating transcription factor 4 and runt-related transcription factor 2.The Journal of biological chemistry · 2008Article
- Role of RUNX in autoimmune diseases linking rheumatoid arthritis, psoriasis and lupus.Arthritis research & therapy · 2004Review
- Regulation of the bone-specific osteocalcin gene by p300 requires Runx2/Cbfa1 and the vitamin D3 receptor but not p300 intrinsic histone acetyltransferase activity.Molecular and cellular biology · 2003Article
- Nuclear microenvironments support physiological control of gene expression.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Review
- Interaction of the 1alpha,25-dihydroxyvitamin D3 receptor at the distal promoter region of the bone-specific osteocalcin gene requires nucleosomal remodelling.The Biochemical journal · 2002Article
- Overexpression of Cbfa1 in osteoblasts inhibits osteoblast maturation and causes osteopenia with multiple fractures.The Journal of cell biology · 2001Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Tissue and cell-type specific expression of the rat osteocalcin (rOC) gene involves the interplay of multiple transcriptional regulatory factors. In this report we demonstrate that AML-1 (acute myeloid leukemia-1), a DNA-binding protein whose genes are disrupted by chromosomal translocations in several human leukemias, interacts with a sequence essential for enhancing tissue-restricted expression of the rOC gene. Deletion analysis of rOC promoter-chloramphenicol acetyltransferase constructs demonstrates that an AML-1-binding sequence within the proximal promoter (-138 to -130 nt) contributes to 75% of the level of osteocalcin gene expression. The activation potential of the AML-1-binding sequence has been established by overexpressing AML-1 in osteoblastic as well as in nonosseous cell lines. Overexpression not only enhances rOC promoter activity in osteoblasts but also mediates OC promoter activity in a nonosseous human fibroblastic cell line. A probe containing this site forms a sequence specific protein-DNA complex with nuclear extracts from osteoblastic cells but not from nonosseous cells. Antisera supershift experiments indicate the presence of AML-1 and its partner protein core-binding factor beta in this osteoblast-restricted complex. Mutations of the critical AML-1-binding nucleotides abrogate formation of the complex and strongly diminish promoter activity. These results indicate that an AML-1 related protein is functional in cells of the osteoblastic lineage and that the AML-1-binding site is a regulatory element important for osteoblast-specific transcriptional activation of the rOC gene.
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