ArticleProceedings of the National Academy of Sciences of the United States of America1996
High resolution ultrastructural mapping of total calcium: electron spectroscopic imaging/electron energy loss spectroscopy analysis of a physically/chemically processed nerve-muscle preparation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 52 citations in OpenAlex.
- Calcium-Sensing Receptor: A Key Target for Extracellular Calcium Signaling in Neurons.Frontiers in physiology · 2016Review
- Iron sources used by the nonpathogenic lactic acid bacterium Lactobacillus sakei as revealed by electron energy loss spectroscopy and secondary-ion mass spectrometry.Applied and environmental microbiology · 2010Article
- A store-operated Ca(2+) influx pathway in the bag cell neurons of Aplysia.Journal of neurophysiology · 2006Article
- Direct visualization of intracellular calcium in rat osteoblasts by energy-filtering transmission electron microscopy.Histochemistry and cell biology · 2004Article
- Differential regulation of transmitter release by presynaptic and glial Ca2+ internal stores at the neuromuscular synapse.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- alpha-Latrotoxin releases calcium in frog motor nerve terminals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Depletion of calcium in the synaptic cleft of a calyx-type synapse in the rat brainstem.The Journal of physiology · 1999Article
- Neurosecretory cells without neurosecretion: evidence of an independently regulated trait of the cell phenotype.The Journal of physiology · 1999Review
- Quantification of total calcium in terminal cisternae of skinned muscle fibers by imaging electron energy-loss spectroscopy.Journal of muscle research and cell motility · 1999Article
- A combined solution exchange/plunge-freezing device for skinned muscle fibers.Journal of muscle research and cell motility · 1999Article
- Overexpression of CALNUC (nucleobindin) increases agonist and thapsigargin releasable Ca2+ storage in the Golgi.The Journal of cell biology · 1999Article
- Presynaptic mitochondria and the temporal pattern of neurotransmitter release.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1999Article
- The frog neuromuscular junction revisited after quick-freezing-freeze-drying: ultrastructure, immunogold labelling and high resolution calcium mapping.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 1999Article
- Multiple and diverse forms of regulated exocytosis in wild-type and defective PC12 cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein.The Journal of cell biology · 1998Article
- High-resolution calcium mapping of the endoplasmic reticulum-Golgi-exocytic membrane system. Electron energy loss imaging analysis of quick frozen-freeze dried PC12 cells.Molecular biology of the cell · 1997Article
- Dynamic properties of an inositol 1,4,5-trisphosphate- and thapsigargin-insensitive calcium pool in mammalian cell lines.The Journal of cell biology · 1997Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on a procedure for tissue preparation that combines thoroughly controlled physical and chemical treatments: quick-freezing and freeze-drying followed by fixation with OsO4 vapors and embedding by direct resin infiltration. Specimens of frog cutaneous pectoris muscle thus prepared were analyzed for total calcium using electron spectroscopic imaging/electron energy loss spectroscopy (ESI/EELS) approach. The preservation of the ultrastructure was excellent, with positive K/Na ratios revealed in the fibers by x-ray microanalysis. Clear, high-resolution EELS/ESI calcium signals were recorded from the lumen of terminal cisternae of the sarcoplasmic reticulum but not from longitudinal cisternae, as expected from previous studies carried out with different techniques. In many mitochondria, calcium was below detection whereas in others it was appreciable although at variable level. Within the motor nerve terminals, synaptic vesicles as well as some cisternae of the smooth endoplasmic reticulum yielded positive signals at variance with mitochondria, that were most often below detection. Taken as a whole, the present study reveals the potential of our experimental approach to map with high spatial resolution the total calcium within individual intracellular organelles identified by their established ultrastructure, but only where the element is present at high levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.