ArticleProceedings of the National Academy of Sciences of the United States of America1996
Single cell Ca2+/cAMP cross-talk monitored by simultaneous Ca2+/cAMP fluorescence ratio imaging.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 37 citations in OpenAlex.
- Integration of cAMP and TRPV4 Signaling to Optimize Collagen Remodeling for Management of Fibrosis.Cells · 2025Review
- Synthesis and properties of Asante Calcium Red--a novel family of long excitation wavelength calcium indicators.Cell calcium · 2013Article
- Epac and PKA: a tale of two intracellular cAMP receptors.Acta biochimica et biophysica Sinica · 2008Review
- Imaging second messenger dynamics in developing neural circuits.Developmental neurobiology · 2008Review
- Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells.Science's STKE : signal transduction knowledge environment · 2006Article
- Interplay of Ca2+ and cAMP signaling in the insulin-secreting MIN6 beta-cell line.The Journal of biological chemistry · 2005Article
- Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signaling within discrete subcellular compartments.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Regulation and organization of adenylyl cyclases and cAMP.The Biochemical journal · 2003Review
- Cyclic nucleotide-gated channels colocalize with adenylyl cyclase in regions of restricted cAMP diffusion.The Journal of general physiology · 2000Article
- cAMP-dependent protein kinase phosphorylates and activates nuclear Ca2+-ATPase.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Characterization of soluble forms of nonchimeric type V adenylyl cyclases.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The spatial and temporal dynamics of two intracellular second messengers, cAMP and Ca2+, were simultaneously monitored in living cells by digital fluorescence ratio imaging using FlCRhR, a single-excitation dual-emission cAMP indicator, and fura-2, a dual-excitation single-emission Ca2+ probe. In single C6-2B glioma cells, isoproterenol- or forskolin-evoked cAMP accumulation (measured in vivo as an increased FlCRhR emission ratio) was reduced when cytosolic free Ca2+ concentration was elevated before, simultaneously with, or after cAMP activation. However, in REF-52 fibroblasts, Ca2+ neither prevented nor reduced forskolin-stimulated cAMP production. These results provide novel in vivo evidence for the Ca2+ modulation of the cAMP transduction pathway in C6-2B cells. The simultaneous microscopic measurement of cAMP and Ca2+ kinetics in single cells makes it now possible to study the regulatory interactions between these second messengers at the cellular and even the subcellular level.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.