ArticleJournal of virology1996
Factors regulating baculovirus late and very late gene expression in transient-expression assays.
Article in Journal of virology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 81 citations in OpenAlex.
- The conserved cysteines at position 18, 36, and 49 of Autographa californica multiple nucleopolyhedrovirus VP39 are essential for virus replication.Virus genes · 2024Article
- Entomopathogenic Viruses in the Neotropics: Current Status and Recently Discovered Species.Neotropical entomology · 2020Review
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- Ha83, a Chitin Binding Domain Encoding Gene, Is Important to Helicoverpa armigera Nucleopolyhedrovirus Budded Virus Production and Occlusion Body Assembling.Scientific reports · 2015Article
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- Baculovirus: molecular insights on their diversity and conservation.International journal of evolutionary biology · 2011Article
- Characterization of an early gene orf122 from Bombyx mori nucleopolyhedrovirus.Molecular biology reports · 2009Article
- Genome sequence of Leucania seperata nucleopolyhedrovirus.Virus genes · 2007Article
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- Transcriptional activity of baculovirus very late factor 1.Journal of virology · 2005Article
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- Baculovirus lef-12 is not required for viral replication.Journal of virology · 2002Article
- Baculovirus infection raises the level of TATA-binding protein that colocalizes with viral DNA replication sites.Journal of virology · 2002Article
- Genetic requirements for homologous recombination in Autographa californica nucleopolyhedrovirus.Journal of virology · 2002Article
- Analysis of an Autographa californica nucleopolyhedrovirus lef-11 knockout: LEF-11 is essential for viral DNA replication.Journal of virology · 2002Article
- Activation of baculovirus very late promoters by interaction with very late factor 1.Journal of virology · 1999Article
- Colocalization of baculovirus IE-1 and two DNA-binding proteins, DBP and LEF-3, to viral replication factories.Journal of virology · 1999Article
- Nineteen baculovirus open reading frames, including LEF-12, support late gene expression.Journal of virology · 1998Article
- In vitro transcription of pe38/polyhedrin hybrid promoters reveals sequences essential for recognition by the baculovirus-induced RNA polymerase and for the strength of very late viral promoters.Journal of virology · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Eighteen genes of Autographa californica nuclear polyhedrosis virus are necessary and sufficient to transactivate expression from the late vp39 promoter in transient-expression assays in SF-21 cells. These 18 genes, known as late expression factor genes (lefs), are also required to transactivate the very late promoter of the polyhedrin gene, polh, but expression from this promoter is relatively weak compared with expression from the vp39 promoter. To further define the factors required for late and very late promoter expression, we first determined that the eighteen lefs were also required for expression from two other major baculovirus promoters: the late basic 6.9-kDa protein gene, p6.9, and the very late 10-kDa protein gene, p10. We next examined the effect of the very late expression factor 1 gene (vlf-1), a gene previously identified by analysis of a temperature-sensitive mutant, in the transient expression assay and found that vlf-1 specifically transactivated the two very late promoters but not the two late promoters. We then surveyed the Autographa californica nuclear polyhedrosis virus genome for additional genes which might specifically regulate very late gene expression; no additional vlf genes were detected, suggesting that VLF-1 is the primary regulator of very late gene expression. Finally, we found that the relative contribution of the antiapoptosis gene p35, which behaves as a lef in these transient-expression assays, depended on the nature of the other viral genes provided in the cotransfection mixtures, suggesting that other viral genes also contribute to the ability of the virus to block apoptosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.