ArticleInfection and immunity1996
A Legionella pneumophila gene that promotes hemin binding.
Article in Infection and immunity, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 52 citations in OpenAlex.
- Major type IV pilin ofmBio · 2026Article
- Molecular evolution and adaptations ofFrontiers in cellular and infection microbiology · 2026Review
- Review
- Legionella pneumophila Rhizoferrin Promotes Bacterial Biofilm Formation and Growth within Amoebae and Macrophages.Infection and immunity · 2023Article
- Mechanisms of heme utilization by Francisella tularensis.PloS one · 2015Article
- An update on iron acquisition by Legionella pneumophila: new pathways for siderophore uptake and ferric iron reduction.Future microbiology · 2015Review
- Role and regulation of heme iron acquisition in gram-negative pathogens.Frontiers in cellular and infection microbiology · 2013Review
- Purification of Legiobactin and importance of this siderophore in lung infection by Legionella pneumophila.Infection and immunity · 2009Article
- The secreted pyomelanin pigment of Legionella pneumophila confers ferric reductase activity.Infection and immunity · 2007Article
- The Legionella pneumophila tatB gene facilitates secretion of phospholipase C, growth under iron-limiting conditions, and intracellular infection.Infection and immunity · 2005Article
- Cloning and characterization of the gene encoding the major cell-associated phospholipase A of Legionella pneumophila, plaB, exhibiting hemolytic activity.Infection and immunity · 2004Article
- Legionella pneumophila feoAB promotes ferrous iron uptake and intracellular infection.Infection and immunity · 2002Article
- The cytochrome c maturation locus of Legionella pneumophila promotes iron assimilation and intracellular infection and contains a strain-specific insertion sequence element.Infection and immunity · 2002Article
- Influence of the alternative sigma(28) factor on virulence and flagellum expression of Legionella pneumophila.Infection and immunity · 2002Article
- Identification of Legionella pneumophila rcp, a pagP-like gene that confers resistance to cationic antimicrobial peptides and promotes intracellular infection.Infection and immunity · 2001Article
- Type II protein secretion is a subset of the PilD-dependent processes that facilitate intracellular infection by Legionella pneumophila.Infection and immunity · 2001Article
- Hemin-binding surface protein from Bartonella quintana.Infection and immunity · 2000Article
- The Legionella pneumophila iraAB locus is required for iron assimilation, intracellular infection, and virulence.Infection and immunity · 2000Article
- Discovery of a nonclassical siderophore, legiobactin, produced by strains of Legionella pneumophila.Journal of bacteriology · 2000Article
- Legionella pneumophila utilizes the same genes to multiply within Acanthamoeba castellanii and human macrophages.Infection and immunity · 1999Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The ability to bind and utilize hemin is a trait common to many human pathogens. Nevertheless, the relationship between Legionella pneumophila, the agent of Legionnaires' disease, and hemin has received little attention. Thus, we explored the capacity of a virulent, serogroup 1 strain of L. pneumophila to bind hemin and use it as an iron source. Hemin, but not protoporphyrin IX, restored bacterial growth in iron-limiting media, indicating that it can serve as an iron source for L. pneumophila. In support of this idea, we observed that wildtype legionellae were able to bind 50 to 60% of added hemin, a binding capacity that was comparable to those of other pathogens. To begin to identify proteins involved in hemin acquisition, we identified a Legionella locus that conferred hemin binding upon Escherichia coli. Subcloning and nucleotide sequence analysis determined that a single open reading frame, which was designated hbp for hemin-binding promotion, was responsible for this binding activity. The hbp gene was predicted to encode a secreted, 15.5-kDa protein. To ascertain the importance of this gene in L. pneumophila biology, we used allelic exchange to construct an hbp mutant. Importantly, the mutant displayed a 42% reduction in hemin binding, confirming that hbp potentiates hemin acquisition by L. pneumophila. However, the strain was unaltered in its ability to grow within macrophage-like cells and freshwater amoebae, indicating that hbp is not required for intracellular infection. Despite this, Southern hybridization analysis and database searches demonstrated that hbp is nearly exclusive to the L. pneumophila species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.