ArticleThe EMBO journal1996
Cooperation at a distance between silencers and proto-silencers at the yeast HML locus.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 80 citations in OpenAlex.
- Regulation of the heterochromatin spreading reaction byOpen biology · 2023Review
- Epigenetic fates of gene silencing established by heterochromatin spreading in cell identity and genome stability.Current genetics · 2019Review
- Article
- Global genome nucleotide excision repair is organized into domains that promote efficient DNA repair in chromatin.Genome research · 2016Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- A protosilencer of subtelomeric gene expression in Candida glabrata with unique properties.Genetics · 2012Article
- Functions of protosilencers in the formation and maintenance of heterochromatin in Saccharomyces cerevisiae.PloS one · 2012Article
- Modeling of chromosomal epigenetic silencing processes.Transcription · 2011Article
- Sub-telomeric core X and Y' elements in S. cerevisiae suppress extreme variations in gene silencing.PloS one · 2011Article
- Role for cohesin in the formation of a heterochromatic domain at fission yeast subtelomeres.Molecular and cellular biology · 2011Article
- A cis-acting tRNA gene imposes the cell cycle progression requirement for establishing silencing at the HMR locus in yeast.Genetics · 2011Article
- Article
- Design of a minimal silencer for the silent mating-type locus HML of Saccharomyces cerevisiae.Nucleic acids research · 2010Article
- Spatial epigenetic control of mono- and bistable gene expression.PLoS biology · 2010Article
- Silent information regulator 3: the Goldilocks of the silencing complex.Genes & development · 2010Review
- The functional importance of telomere clustering: global changes in gene expression result from SIR factor dispersion.Genome research · 2009Article
- Regulation of nuclear positioning and dynamics of the silent mating type loci by the yeast Ku70/Ku80 complex.Molecular and cellular biology · 2009Article
- Subtelomeric ACS-containing proto-silencers act as antisilencers in replication factors mutants in Saccharomyces cerevisiae.Molecular biology of the cell · 2009Article
- Long-range communication between the silencers of HMR.Molecular and cellular biology · 2008Article
- Conversion of a replication origin to a silencer through a pathway shared by a Forkhead transcription factor and an S phase cyclin.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcriptional repression at the silent yeast mating type loci is achieved through the formation of a particular nucleoprotein complex at specific cis-acting elements called silencers. This complex in turn appears to initiate the spreading of a histone binding protein complex into the surrounding chromatin, which restricts accessibility of the region to the transcription machinery. We have investigated long-range, cooperative effects between silencers by studying the repression of a reporter gene integrated at the HML locus flanked by various combinations of wild-type and mutated silencer sequences. Two silencers can cooperate over >4000 bp to repress transcription efficiently. More importantly, a single binding site for either the repressor activator protein 1 (Rap1), the autonomous replicating sequence (ARS) binding factor 1 (Abf1) or the origin recognition complex (ORC) can enhance the action of a distant silencer without acting as a silencer on its own. Functional cooperativity is demonstrated using a quantitative assay for repression, and varies with the affinity of the binding sites used. Since the repression mechanism is Sir dependent, the Rap1, ORC and/or Abf1 proteins bound to distant DNA elements may interact to create an interface of sufficiently high affinity such that Sir-containing complexes bind, nucleating the silent chromatin state.
Indexed as
Identifiers
8641284PMC450142W2223638669What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.