ArticleThe EMBO journal1996
Implications for the domain arrangement of axonin-1 derived from the mapping of its NgCAM binding site.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
25 citing papers in PubMed, 74 citations in OpenAlex.
- Molecular mechanism of contactin 2 homophilic interaction.Structure (London, England : 1993) · 2024Article
- Spatiotemporal changes in Netrin/Dscam1 signaling dictate axonal projection direction ineLife · 2024Article
- Structural insights into the contactin 1 - neurofascin 155 adhesion complex.Nature communications · 2022Article
- Functional Diversity of Neuronal Cell Adhesion and Recognition Molecule L1CAM through Proteolytic Cleavage.Cells · 2022Review
- Regulating Axonal Responses to Injury: The Intersection between Signaling Pathways Involved in Axon Myelination and The Inhibition of Axon Regeneration.Frontiers in molecular neuroscience · 2016Review
- Review
- Regulation of adhesion by flexible ectodomains of IgCAMs.Neurochemical research · 2013Review
- TAG1 regulates the endocytic trafficking and signaling of the semaphorin3A receptor complex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Inside-out regulation of L1 conformation, integrin binding, proteolysis, and concomitant cell migration.Molecular biology of the cell · 2010Article
- Lewis(x) and alpha2,3-sialyl glycans and their receptors TAG-1, Contactin, and L1 mediate CD24-dependent neurite outgrowth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Structural requirement of TAG-1 for retinal ganglion cell axons and myelin in the mouse optic nerve.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- The crystal structure of the ligand-binding module of human TAG-1 suggests a new mode of homophilic interaction.Protein science : a publication of the Protein Society · 2007Article
- The role of cytoplasmic serine residues of the cell adhesion molecule L1 in neurite outgrowth, endocytosis, and cell migration.Cellular and molecular neurobiology · 2007Article
- The neuronal adhesion protein TAG-1 is expressed by Schwann cells and oligodendrocytes and is localized to the juxtaparanodal region of myelinated fibers.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- Cell adhesion molecule L1 in folded (horseshoe) and extended conformations.Molecular biology of the cell · 2001Article
- A direct interaction of axonin-1 with NgCAM-related cell adhesion molecule (NrCAM) results in guidance, but not growth of commissural axons.The Journal of cell biology · 2000Article
- Defining a role and mechanism for IgCAM function in vertebrate axon guidance.The Journal of cell biology · 2000Article
- Neurolin Ig domain 2 participates in retinal axon guidance and Ig domains 1 and 3 in fasciculation.The Journal of cell biology · 1999Article
- Neurite fasciculation mediated by complexes of axonin-1 and Ng cell adhesion molecule.The Journal of cell biology · 1998Article
- Dissection of complex molecular interactions of neurofascin with axonin-1, F11, and tenascin-R, which promote attachment and neurite formation of tectal cells.The Journal of cell biology · 1998Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The neuronal cell adhesion molecule axonin-1 is composed of six immunoglobulin and four fibronectin type III domains. Axonin-1 promotes neurite outgrowth, when presented as a substratum for neurons in vitro, via a neuronal receptor that has been identified as the neuron-glia cell adhesion molecule, NgCAM, based on the blocking effect of polyclonal antibodies directed to NgCAM. Here we report the identification of axonin-1 domains involved in NgCAM binding. NgCAM-conjugated microspheres were tested for binding to COS cells expressing domain deletion mutants of axonin-1. In addition, monoclonal antibodies directed to axonin-1 were assessed for their ability to block the axonin-1-NgCAM interaction, and their epitopes were mapped using the domain deletion mutants. The results suggest that the four amino-terminal immunoglobulin domains of axonin-1 form a domain conglomerate which is necessary and sufficient for NgCAM binding. Surprisingly, NgCAM binding to membrane-bound axonin-1 was increased strongly by deletion of the fifth or sixth immunoglobulin domains of axonin-1. Based on these results and on negative staining electron microscopy, we propose a horseshoe-shaped domain arrangement of axonin-1 that obscures the NgCAM binding site. Neurite outgrowth studies with truncated forms of axonin-1 show that axonin-1 is a neurite outgrowth-promoting substratum in the absence of the NgCAM binding site.
Indexed as
Identifiers
8641271PMC450127W2297808440What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.