ArticleProceedings of the National Academy of Sciences of the United States of America1996
Multiplex genotype determination at a large number of gene loci.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Genetic Association of Pulmonary Surfactant Protein Genes, SFTPA1, SFTPA2, SFTPB, SFTPC, and SFTPD With Cystic Fibrosis.Frontiers in immunology · 2018Article
- Genetic association and epistatic interaction of the interleukin-10 signaling pathway in pediatric inflammatory bowel disease.World journal of gastroenterology · 2017Article
- Read count-based method for high-throughput allelic genotyping of transposable elements and structural variants.BMC genomics · 2015Article
- NOD2 mutations affect muramyl dipeptide stimulation of human B lymphocytes and interact with other IBD-associated genes.Digestive diseases and sciences · 2013Article
- Genetic variants and monoallelic expression of surfactant protein-D in inflammatory bowel disease.Annals of human genetics · 2011Article
- A method for counting PCR template molecules with application to next-generation sequencing.Nucleic acids research · 2011Article
- Genetic association of nonsynonymous variants of the IL23R with familial and sporadic inflammatory bowel disease in women.Digestive diseases and sciences · 2010Article
- Expanded genetic alphabets in the polymerase chain reaction.Angewandte Chemie (International ed. in English) · 2010Article
- A ligation assay for multiplex analysis of CpG methylation using bisulfite-treated DNA.Nucleic acids research · 2007Article
- Influence of language and ancestry on genetic structure of contiguous populations: a microsatellite based study on populations of Orissa.BMC genetics · 2005Article
- A genotyping system capable of simultaneously analyzing >1000 single nucleotide polymorphisms in a haploid genome.Genome research · 2005Article
- Deciphering diversity in populations of various linguistic and ethnic affiliations of different geographical regions of India: analysis based on 15 microsatellite markers.Journal of genetics · 2004Article
- Direct detection of insertion/deletion polymorphisms in an autosomal region by analyzing high-density markers in individual spermatozoa.American journal of human genetics · 2002Article
- Multiplex loss of heterozygosity analysis by using single or very few cells.The Journal of molecular diagnostics : JMD · 2002Article
- Multiplex genotype analysis of invasive carcinoma and accompanying proliferative lesions microdissected from breast tissue.The Journal of molecular diagnostics : JMD · 2000Article
- Determination of gene organization in individual haplotypes by analyzing single DNA fragments from single spermatozoa.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Article
- Efficacy of Monitoring Platelet Function by an Automated PL-12 Analyzer During the Treatment of Acute Cerebral Infarction With Antiplatelet Medicine.Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/HemostasisArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To facilitate large-scale genotype analysis, an efficient PCR-based multiplex approach has been developed. For simultaneously amplifying the target sequences at a large number of genetic loci, locus-specific primers containing 5' universal tails are used. Attaching the universal tails to the target sequences in the initial PCR steps allows replacement of all specific primers with a pair of primers identical to the universal tails and converts the multiplex amplification into "uniplex." Simultaneous amplification of 26 genetic loci with this approach is described. The multiplex amplification can be coupled with genotype determination. By incorporating a single-base mismatch between a primer and the template into the target sequences, a polymorphic site can be converted into a desirable restriction fragment length polymorphism when it is necessary. In this way, the allelic PCR products for the polymorphic loci can be discriminated by gel electrophoresis after restriction enzyme digestion. In this study, 32 loci were typed in such a multiplex way.
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