ArticleProceedings of the National Academy of Sciences of the United States of America1996
A long-range regulatory element of Hoxc8 identified by using the pClasper vector.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- The crest phenotype in chicken is associated with ectopic expression of HOXC8 in cranial skin.PloS one · 2012Article
- Multiple promoters and alternative splicing: Hoxa5 transcriptional complexity in the mouse embryo.PloS one · 2010Article
- Identification and analysis of a conserved Tcfap2a intronic enhancer element required for expression in facial and limb bud mesenchyme.Molecular and cellular biology · 2008Article
- Transgenic LacZ under control of Hec-6st regulatory sequences recapitulates endogenous gene expression on high endothelial venules.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Distinct regulatory elements mediate the dynamic expression pattern of Nkx3.1.Developmental dynamics : an official publication of the American Association of Anatomists · 2005Article
- The identification of Hoxc8 target genes.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Cdx protein interaction with Hoxa5 regulatory sequences contributes to Hoxa5 regional expression along the axial skeleton.Molecular and cellular biology · 2005Article
- Divergence of Hoxc8 early enhancer parallels diverged axial morphologies between mammals and fishes.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Genomic structure and functional control of the Dlx3-7 bigene cluster.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Isolation and characterization of BEN, a member of the TFII-I family of DNA-binding proteins containing distinct helix-loop-helix domains.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Mammalian Trithorax and polycomb-group homologues are antagonistic regulators of homeotic development.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Comparative studies on mammalian Hoxc8 early enhancer sequence reveal a baleen whale-specific deletion of a cis-acting element.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Transfer of P1 inserts into a yeast-bacteria shuttle vector by co-transformation mediated homologous recombination.Nucleic acids research · 1998Article
- Modification of expression and cis-regulation of Hoxc8 in the evolution of diverged axial morphology.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Combinations of closely situated cis-acting elements determine tissue-specific patterns and anterior extent of early Hoxc8 expression.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Functional specificity of Hoxa-4 in vertebral patterning lies outside of the homeodomain.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Hox genes are located in highly conserved clusters. The significance of this organization is unclear, but one possibility is that regulatory regions for individual genes are dispersed throughout the cluster and shared with other Hox genes. This hypothesis is supported by studies on several Hox genes in which even large genomic regions immediately surrounding the gene fail to direct the complete expression pattern in transgenic mice. In particular, previous studies have identified proximal regulatory regions that are primarily responsible for early phases of mouse Hoxc8 expression. To locate additional regulatory regions governing expression during the later periods of development, a yeast homologous recombination-based strategy utilizing the pClasper vector was employed. Using homologous recombination into pClasper, we cloned a 27-kb region around the Hoxc8 gene from a yeast artificial chromosome. A reporter gene was introduced into the coding region of the isolated gene by homologous recombination in yeast. This large fragment recapitulates critical aspects of Hoxc8 expression in transgenic mice. We show that the regulatory elements that maintain the anterior boundaries of expression in the neural tube and paraxial mesoderm are located between 11 and 19 kb downstream of the gene.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.