ArticleThe Journal of cell biology1996
Cell cycle regulation of dynein association with membranes modulates microtubule-based organelle transport.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 163 citations in OpenAlex.
- Cell cycle-regulated tug-of-war between microtubule motors positions major trafficking organelles.bioRxiv : the preprint server for biology · 2025Article
- CDR2 is a dynein adaptor recruited by kinectin to regulate ER sheet organization.The Journal of cell biology · 2025Article
- The meiotic LINC complex component KASH5 is an activating adaptor for cytoplasmic dynein.The Journal of cell biology · 2023Article
- Cytoplasmic dynein1 intermediate-chain2 regulates cellular trafficking and physiopathological development iniScience · 2023Article
- Differential contribution of nuclear size scaling mechanisms between Xenopus species.Development, growth & differentiation · 2022Article
- Intertwined and Finely Balanced: Endoplasmic Reticulum Morphology, Dynamics, Function, and Diseases.Cells · 2021Review
- Network organisation and the dynamics of tubules in the endoplasmic reticulum.Scientific reports · 2021Article
- Exploring the Role of a Cytokinin-Activating Enzyme LONELY GUY in Unicellular MicroalgaFrontiers in plant science · 2020Article
- A novel, dynein-independent mechanism focuses the endoplasmic reticulum around spindle poles in dividing Drosophila spermatocytes.Scientific reports · 2019Article
- LIM kinase1 regulates mitotic centrosome integrity via its activity on dynein light intermediate chains.Open biology · 2018Article
- Phosphorylation-Induced Motor Shedding Is Required at Mitosis for Proper Distribution and Passive Inheritance of Mitochondria.Cell reports · 2016Article
- GM130 Regulates Golgi-Derived Spindle Assembly by Activating TPX2 and Capturing Microtubules.Cell · 2015Article
- Distinct functional roles of cytoplasmic dynein defined by the intermediate chain isoforms.Experimental cell research · 2015Review
- Dynein light intermediate chains maintain spindle bipolarity by functioning in centriole cohesion.The Journal of cell biology · 2014Article
- Bidirectional cargo transport: moving beyond tug of war.Nature reviews. Molecular cell biology · 2014Review
- Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84.Journal of neuroscience research · 2014Article
- The basal position of nuclei is one pre-requisite for asymmetric cell divisions in the early mouse embryo.Developmental biology · 2014Article
- PKA-dependent dynein switching from lysosomes to adenovirus: a novel form of host-virus competition.The Journal of cell biology · 2014Article
- Multiple mechanisms determine ER network morphology during the cell cycle in Xenopus egg extracts.The Journal of cell biology · 2013Article
- Biased inheritance of mitochondria during asymmetric cell division in the mouse oocyte.Journal of cell science · 2013Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cytoplasmic dynein is a minus end-directed microtubule motor that performs distinct functions in interphase and mitosis. In interphase, dynein transports organelles along microtubules, whereas in metaphase this motor has been implicated in mitotic spindle formation and orientation as well as chromosome segregation. The manner in which dynein activity is regulated during the cell cycle, however, has not been resolved. In this study, we have examined the mechanism by which organelle transport is controlled by the cell cycle in extracts of Xenopus laevis eggs. Here, we show that photocleavage of the dynein heavy chain dramatically inhibits minus end-directed organelle transport and that purified dynein restores this motility, indicating that dynein is the predominant minus end-directed membrane motor in Xenopus egg extracts. By measuring the amount of dynein associated with isolated membranes, we find that cytoplasmic dynein and its activator dynactin detach from the membrane surface in metaphase extracts. The sevenfold decrease in membrane-associated dynein correlated well with the eightfold reduction in minus end-directed membrane transport observed in metaphase versus interphase extracts. Although dynein heavy or intermediate chain phosphorylation did not change in a cell cycle-dependent manner, the dynein light intermediate chain incorporated approximately 12-fold more radiolabeled phosphate in metaphase than in interphase extracts. These studies suggest that cell cycle-dependent phosphorylation of cytoplasmic dynein may regulate organelle transport by modulating the association of this motor with membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.