ArticleThe Journal of cell biology1996
RAN/TC4 mutants identify a common requirement for snRNP and protein import into the nucleus.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 117 citations in OpenAlex.
- PCV2 Cap protein nuclear import via importin α/β receptor: molecular insights and antiviral potential.Frontiers in microbiology · 2025Article
- WW domains form a folded type of nuclear localization signal to guide YAP1 nuclear import.The Journal of cell biology · 2024Article
- Ran-GTP/-GDP-dependent nuclear accumulation of NONEXPRESSOR OF PATHOGENESIS-RELATED GENES1 and TGACG-BINDING FACTOR2 controls salicylic acid-induced leaf senescence.Plant physiology · 2022Article
- Modulation of innate immune signaling by aProceedings of the National Academy of Sciences of the United States of America · 2020Article
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- RanGTP and importin β regulate meiosis I spindle assembly and function in mouse oocytes.The EMBO journal · 2020Article
- Identification of a putative nuclear localization signal in the tumor suppressor maspin sheds light on its nuclear import regulation.FEBS open bio · 2019Article
- Article
- Karyopherin α-3 is a key protein in the pathogenesis of spinocerebellar ataxia type 3 controlling the nuclear localization of ataxin-3.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Structural analysis of the role of TPX2 in branching microtubule nucleation.The Journal of cell biology · 2017Article
- Probing the nuclear import signal and nuclear transport molecular determinants of PRV ICP22.Cell & bioscience · 2016Article
- Nuclear localization signals of varicella zoster virus ORF4.Virus genes · 2014Article
- Probing of the nuclear import and export signals and subcellular transport mechanism of varicella-zoster virus tegument protein open reading frame 10.Medical microbiology and immunology · 2012Article
- HIV-1 accessory protein Vpr: relevance in the pathogenesis of HIV and potential for therapeutic intervention.Retrovirology · 2011Review
- Dynamics of the STAT3 transcription factor: nuclear import dependent on Ran and importin-β1.PloS one · 2011Article
- Molecular anatomy of subcellular localization of HSV-1 tegument protein US11 in living cells.Virus research · 2010Article
- Interaction between Poly(ADP-ribose) and NuMA contributes to mitotic spindle pole assembly.Molecular biology of the cell · 2009Article
- The ins and outs of nuclear trafficking: unusual aspects in apicomplexan parasites.DNA and cell biology · 2009Review
- The dynamics and mechanisms of interleukin-1alpha and beta nuclear import.Traffic (Copenhagen, Denmark) · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinetic competition experiments have demonstrated that at least some factors required for the nuclear import of proteins and U snRNPs are distinct. Both import processes require energy, and in the case of protein import, the energy requirement is known to be at least partly met by GTP hydrolysis by the Ran GTPase. We have compared the effects of nonhydrolyzable GTP analogues and two mutant Ran proteins on the nuclear import of proteins and U snRNPs in vitro. The mutant Ran proteins have different defects; Q69L (glutamine 69 changed to leucine) is defective in GTP hydrolysis while T24N (threonine 24 changed to asparagine) is defective in binding GTP. Both protein and snRNP import are sensitive either to the presence of the two mutant Ran proteins, which act as dominant negative inhibitors of nuclear import, or to incubation with nonhydrolyzable GTP analogues. This demonstrates that there is a requirement for a GTPase activity for the import of U snRNPs, as well as proteins, into the nucleus. The dominant negative effects of the two mutant Ran proteins indicate that the pathways of protein and snRNP import share at lease one common component.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.