ArticleThe Journal of cell biology1996
Removal of MAP4 from microtubules in vivo produces no observable phenotype at the cellular level.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 71 citations in OpenAlex.
- MAP4 as a New Candidate in Cardiovascular Disease.Frontiers in physiology · 2020Review
- Regulation of microtubule-based transport by MAP4.Molecular biology of the cell · 2014Article
- The role of microtubules and their dynamics in cell migration.The Journal of biological chemistry · 2012Article
- Highly dynamic microtubules improve the effectiveness of early stages of human influenza A/NWS/33 virus infection in LLC-MK2 cells.PloS one · 2012Article
- New insights into mechanisms of resistance to microtubule inhibitors.Biochimica et biophysica acta · 2011Review
- MAP4 and CLASP1 operate as a safety mechanism to maintain a stable spindle position in mitosis.Nature cell biology · 2011Article
- Class V β-tubulin alters dynamic instability and stimulates microtubule detachment from centrosomes.Molecular biology of the cell · 2011Article
- Megakaryocyte lineage-specific class VI β-tubulin suppresses microtubule dynamics, fragments microtubules, and blocks cell division.Cytoskeleton (Hoboken, N.J.) · 2011Article
- Inhibition of cell migration and cell division correlates with distinct effects of microtubule inhibiting drugs.The Journal of biological chemistry · 2010Article
- Predominant regulators of tubulin monomer-polymer partitioning and their implication for cell polarization.Cellular and molecular life sciences : CMLS · 2009Review
- Interphase-specific phosphorylation-mediated regulation of tubulin dimer partitioning in human cells.Molecular biology of the cell · 2007Article
- CaMKIIgamma-mediated inactivation of the Kin I kinesin MCAK is essential for bipolar spindle formation.The EMBO journal · 2005Article
- The MAP2/Tau family of microtubule-associated proteins.Genome biology · 2005Review
- Regulation of microtubule dynamics and myogenic differentiation by MURF, a striated muscle RING-finger protein.The Journal of cell biology · 2000Article
- Differentiation of human colon cancer cells changes the expression of beta-tubulin isotypes and MAPs.British journal of cancer · 1999Article
- Acute inactivation of tau has no effect on dynamics of microtubules in growing axons of cultured sympathetic neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Nonneuronal isoforms of STOP protein are responsible for microtubule cold stability in mammalian fibroblasts.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Microtubule stabilization in pressure overload cardiac hypertrophy.The Journal of cell biology · 1997Article
- XMAP310: a Xenopus rescue-promoting factor localized to the mitotic spindle.The Journal of cell biology · 1997Article
- Mitogen-activated protein kinase/extracellular signal-regulated kinase 2 regulates cytoskeletal organization and chemotaxis via catalytic and microtubule-specific interactions.Molecular biology of the cell · 1997Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Microtubule-associated protein 4 (MAP4) promotes MT assembly in vitro and is localized along MTs in vivo. These results and the fact that MAP4 is the major MAP in nonneuronal cells suggest that MAP4's normal functions may include the stabilization of MTs in situ. To understand MAP4 function in vivo, we produced a blocking antibody (Ab) to prevent MAP4 binding to MTs. The COOH-terminal MT binding domain of MAP4 was expressed in Escherichia coli as a glutathione transferase fusion protein and was injected into rabbits to produce an antiserum that was then affinity purified and shown to be monospecific for MAP4. This Ab blocked > 95% of MAP4 binding to MTs in an in vitro assay. Microinjection of the affinity purified Ab into human fibroblasts and monkey epithelial cells abolished MAP4 binding to MTs as assayed with a rat polyclonal antibody against the NH2-terminal projection domain of MAP4. The removal of MAP4 from MTs was accompanied by its sequestration into visible MAP4-Ab immunocomplexes. However, the MT network appeared normal. Tubulin photoactivation and nocodazole sensitivity assays indicated that MT dynamics were not altered detectably by the removal of MAP4 from the MTs. Cells progressed to mitosis with morphologically normal spindles in the absence of MAP4 binding to MTs. Depleting MAP4 from MTs also did not affect the state of posttranslational modifications of tubulin subunits. Further, no perturbations of MT-dependent organelle distribution were detected. We conclude that the association of MAP4 with MTs is not essential for MT assembly or for the MT-based functions in cultured cells that we could assay. A significant role for MAP4 is not excluded by these results, however, as MAP4 may be a component of a functionally redundant system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.