ArticleThe Journal of cell biology1996
Import of a DHFR hybrid protein into glycosomes in vivo is not inhibited by the folate-analogue aminopterin.
Article in The Journal of cell biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 89 citations in OpenAlex.
- Antifolate agent aminopterin demonstrates potent anti-monkeypox virus activityMicrobiology spectrum · 2026Article
- Structure, Properties, and Function of Glycosomes inFrontiers in cellular and infection microbiology · 2020Review
- Article
- The non-canonical mitochondrial inner membrane presequence translocase of trypanosomatids contains two essential rhomboid-like proteins.Nature communications · 2016Article
- Stage-dependent expression and up-regulation of trypanothione synthetase in amphotericin B resistant Leishmania donovani.PloS one · 2014Article
- The silicon trypanosome: a test case of iterative model extension in systems biology.Advances in microbial physiology · 2014Review
- Explicit consideration of topological and parameter uncertainty gives new insights into a well-established model of glycolysis.The FEBS journal · 2013Article
- Peroxisomal protein import and ERAD: variations on a common theme.Nature reviews. Molecular cell biology · 2010Article
- Characterization and developmentally regulated localization of the mitochondrial carrier protein homologue MCP6 from Trypanosoma brucei.Eukaryotic cell · 2006Article
- Visualization of the ER-to-cytosol dislocation reaction of a type I membrane protein.The EMBO journal · 2002Article
- Acyl-CoA oxidase is imported as a heteropentameric, cofactor-containing complex into peroxisomes of Yarrowia lipolytica.The Journal of cell biology · 2002Article
- Saccharomyces cerevisiae pex3p and pex19p are required for proper localization and stability of peroxisomal membrane proteins.The EMBO journal · 2000Article
- Compartmentation of phosphoglycerate kinase in Trypanosoma brucei plays a critical role in parasite energy metabolism.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The surprising complexity of peroxisome biogenesis.Plant molecular biology · 1998Review
- Pex20p of the yeast Yarrowia lipolytica is required for the oligomerization of thiolase in the cytosol and for its targeting to the peroxisome.The Journal of cell biology · 1998Article
- Pex19p, a farnesylated protein essential for peroxisome biogenesis.Molecular and cellular biology · 1998Article
- Overexpression of Pex15p, a phosphorylated peroxisomal integral membrane protein required for peroxisome assembly in S.cerevisiae, causes proliferation of the endoplasmic reticulum membrane.The EMBO journal · 1997Article
- Enlarged peroxisomes are present in oleic acid-grown Yarrowia lipolytica overexpressing the PEX16 gene encoding an intraperoxisomal peripheral membrane peroxin.The Journal of cell biology · 1997Article
- Oilseed isocitrate lyases lacking their essential type 1 peroxisomal targeting signal are piggybacked to glyoxysomes.The Plant cell · 1997Article
- Identification of Pex13p a peroxisomal membrane receptor for the PTS1 recognition factor.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dihydrofolate reductase fusion proteins have been widely used to study conformational properties of polypeptides translocated across membranes. We have studied the import of dihydrofolate reductase fusion proteins into glycosomes and mitochondria of Trypanosoma brucei. As signal sequences we used the last 22 carboxy-terminal amino acids of glycosomal phosphoglycerate kinase for glycosomes, and the cleavable presequences of yeast cytochrome b2 or cytochrome oxidase subunit IV for mitochondria. Upon addition of aminopterin, a folate analogue that stabilizes the dihydrofolate reductase moiety, import of the fusion protein targeted to glycosomes was not inhibited, although the results of protease protection assays showed that the fusion protein could bind the drug. Under the same conditions, import of a DHFR fusion protein targeted to mitochondria was inhibited by aminopterin. When DHFR fusion proteins targeted simultaneously to both glycosomes and mitochondria were expressed, import into mitochondria was inhibited by aminopterin, whereas uptake of the same proteins into glycosomes was either unaffected or slightly increased. These findings suggest that the glycosomes possess either a strong unfolding activity or an unusually large or flexible translocation channel.
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