ArticleThe EMBO journal1996
Linking microfilaments to intracellular membranes: the actin-binding and vesicle-associated protein comitin exhibits a mannose-specific lectin activity.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 64 citations in OpenAlex.
- Actin acting at the Golgi.Histochemistry and cell biology · 2013Review
- Structural determinants for activity and specificity of the bacterial toxin LlpA.PLoS pathogens · 2013Article
- Phosphorylation of CRN2 by CK2 regulates F-actin and Arp2/3 interaction and inhibits cell migration.Scientific reports · 2012Article
- A Coronin7 homolog with functions in actin-driven processes.The Journal of biological chemistry · 2010Article
- Linking Ras to myosin function: RasGEF Q, a Dictyostelium exchange factor for RasB, affects myosin II functions.The Journal of cell biology · 2008Article
- The liverwort Marchantia polymorpha expresses orthologs of the fungal Agaricus bisporus agglutinin family.Plant physiology · 2007Article
- The identification of inducible cytoplasmic/nuclear carbohydrate-binding proteins urges to develop novel concepts about the role of plant lectins.Glycoconjugate journal · 2004Review
- Plant lectin-like bacteriocin from a rhizosphere-colonizing Pseudomonas isolate.Journal of bacteriology · 2003Article
- Loss of the F-actin binding and vesicle-associated protein comitin leads to a phagocytosis defect.Eukaryotic cell · 2002Article
- Functions of LIM proteins in cell polarity and chemotactic motility.The EMBO journal · 2002Article
- The liverwort contains a lectin that is structurally and evolutionary related to the monocot mannose-binding lectins.Plant physiology · 2002Article
- The Cdk5-p35 kinase associates with the Golgi apparatus and regulates membrane traffic.EMBO reports · 2001Article
- Plant lectins: occurrence, biochemistry, functions and applications.Glycoconjugate journal · 2001Review
- Interaptin, an actin-binding protein of the alpha-actinin superfamily in Dictyostelium discoideum, is developmentally and cAMP-regulated and associates with intracellular membrane compartments.The Journal of cell biology · 1998Article
- Cytoskeletal protein ABP-280 directs the intracellular trafficking of furin and modulates proprotein processing in the endocytic pathway.The Journal of cell biology · 1997Article
- Transfer of free polymannose-type oligosaccharides from the cytosol to lysosomes in cultured human hepatocellular carcinoma HepG2 cells.The Journal of cell biology · 1997Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comitin is a 24 kDa actin-binding protein from Dictyostelium discoideum that is located primarily on Golgi and vesicle membranes. We have probed the molecular basis of comitin's interaction with both actin and membranes using a series of truncation mutants obtained by expressing the appropriate cDNA in Escherichia coli. Comitin dimerizes in solution; its principle actin-binding activity is located between residues 90 and 135. The N-terminal 135 'core' residues of comitin contain a 3-fold sequence repeat that is homologous to several monocotyledon lectins and which retains key residues that determine these lectins' three-dimensional structure and mannose binding. These repeats of comitin appear to mediate its interaction with mannose residues in glycoproteins or glycolipids on the cytoplasmic surface of membrane vesicles from D.discoideum, and comitin can be released from membranes with mannose. Our data indicate that comitin binds to vesicle membranes via mannose residues and, by way of its interaction with actin, links these membranes to the cytoskeleton.
Indexed as
Identifiers
8635456PMC450026W128398215What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.