ArticleCell motility and the cytoskeleton1996
Actin-based motility of isolated axoplasmic organelles.
Article in Cell motility and the cytoskeleton, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- The Giant Axon of the Squid: A Simple System for Axonal Transport Studies.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Fast anterograde transport of herpes simplex virus: role for the amyloid precursor protein of alzheimer's disease.Aging cell · 2003Article
- The role of the cytoskeleton in the life cycle of viruses and intracellular bacteria: tracks, motors, and polymerization machines.Current drug targets. Infectious disorders · 2002Article
- Association of a nonmuscle myosin II with axoplasmic organelles.Molecular biology of the cell · 2002Article
- Microsequencing of myosins for PCR primer design.Methods in molecular biology (Clifton, N.J.) · 2001Review
- Squid axoplasm supports the retrograde axonal transport of herpes simplex virus.The Biological bulletin · 1999Article
- Association of actin filaments with axonal microtubule tracts.Journal of neurocytology · 1999Article
- Unconventional myosins at the crossroad of signal transduction and cytoskeleton remodeling.Protoplasma · 1999Article
- Primary peptide sequences from squid muscle and optic lobe myosin IIs: a strategy to identify an organelle myosin.Cell biology international · 1998Article
- Kinesin- and myosin-driven steps of vesicle recruitment for Ca2+-regulated exocytosis.The Journal of cell biology · 1997Article
- An axoplasmic myosin with a calmodulin-like light chain.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
We previously showed that axoplasmic organelles from the squid giant axon move toward the barbed ends of actin filaments and that KI-washed organelles separated from soluble proteins by sucrose density fractionation retain a 235-kDa putative myosin. Here, we examine the myosin-like activities of KI-washed organelles after sucrose density fractionation to address the question whether the myosin on these organelles is functional. By electron microscopy KI-washed organelles bound to actin filaments in the absence of ATP but not in its presence. Analysis of organelle-dependent ATPase activity over time and with varying amounts of organelles revealed a basal activity of 350 (range: 315-384) nmoles Pi/mg/min and an actin-activated activity of 774 (range: 560-988) nmoles/mg/min, a higher specific activity than for the other fractions. By video microscopy washed organelles moved in only one direction on actin filaments with a net velocity of 1.11 +/- .03 microns/s and an instantaneous velocity of 1.63 +/- 0.29 microns/s. By immunogold electronmicroscopy, 7% of KI-washed organelles were decorated with an anti-myosin antibody as compared to 0.5% with non-immune serum. Thus, some axoplasmic organelles have a tightly associated myosin-like activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.