ArticleApplied and environmental microbiology1996
Application of the novel nucleic acid dyes YOYO-1, YO-PRO-1, and PicoGreen for flow cytometric analysis of marine prokaryotes.
Article in Applied and environmental microbiology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 179 citations in OpenAlex.
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- The Archaeal Cell Cycle.Annual review of cell and developmental biology · 2024Review
- Engineering Planar Gram-Negative Outer Membrane Mimics Using Bacterial Outer Membrane Vesicles.bioRxiv : the preprint server for biology · 2024Article
- Counting Chromosomes in Individual Bacteria to Quantify Their Impacts on Persistence.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Antimicrobial Activity and Proposed Action Mechanism of 3-Carene againstMolecules (Basel, Switzerland) · 2019Article
- PEGylated enhanced cell penetrating peptide nanoparticles for lung gene therapy.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Daily variation in the prokaryotic community during a spring bloom in shelf waters of the East China Sea.FEMS microbiology ecology · 2018Article
- Flow Virometry: a Powerful Tool To Functionally Characterize Viruses.Journal of virology · 2018Review
- Salinity Drives the Virioplankton Abundance but Not Production in Tropical Coastal Lagoons.Microbial ecology · 2018Article
- Article
- Microbial Community Profile and Water Quality in a Protected Area of the Caatinga Biome.PloS one · 2016Article
- Terminal PEGylated DNA-Gold Nanoparticle Conjugates Offering High Resistance to Nuclease Degradation and Efficient Intracellular Delivery of DNA Binding Agents.ACS applied materials & interfaces · 2015Article
- The chromosome copy number of the hyperthermophilic archaeon Thermococcus kodakarensis KOD1.Extremophiles : life under extreme conditions · 2015Article
- Size-independent symmetric division in extraordinarily long cells.Nature communications · 2014Article
- Promoter activity dynamics in the lag phase of Escherichia coli.BMC systems biology · 2013Article
- Characterization of the nucleoid-associated protein YejK.The Journal of biological chemistry · 2013Article
- The cell cycle of archaea.Nature reviews. Microbiology · 2013Review
- Quantitative determination of Plasmodium parasitemia by flow cytometry and microscopy.Journal of Korean medical science · 2012Article
- Modeling the winter-to-summer transition of prokaryotic and viral abundance in the Arctic Ocean.PloS one · 2012Article
- Feasibility of flow cytometry for measurements of Plasmodium falciparum parasite burden in studies in areas of malaria endemicity by use of bidimensional assessment of YOYO-1 and autofluorescence.Journal of clinical microbiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Novel blue light-excited fluorescent dyes for nucleic acids (YOYO-1, YO-PRO-1, and PicoGreen) were tested on cultures of Escherichia coli and of a variety of marine prokaryotes. Results of flow cytometric DNA analyses were compared with those obtained with the UV-excited dyes bis-benzimide Hoechst 33342 or 4', 6-diamidino-2-phenylindole (DAPI). YOYO-1, YO-PRO-1, and PicoGreen can be used only on aldehyde-fixed cells and need to be supplemented with cofactors such as potassium, citrate, or EDTA. They are highly sensitive to ionic strength. Consequently, seawater culture samples cannot be stained directly with these dyes and require at least a 10-fold dilution with distilled water to obtain reliable fluorescence signals. After treatment with RNase, coefficients of variation for the G1 peak of the DNA distributions of the different strains tested with YOYO-1 or PicoGreen indicated in general an improvement over Hoechst 33342 staining. These novel dyes can be used to enumerate prokaryotic cells by flow cytometry, as demonstrated with E. coli. However, their sensitivity to ionic strength makes them unsuitable for cell cycle analysis in natural samples.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.