ArticleThe EMBO journal1996
Retinoic acid-mediated down-regulation of Oct3/4 coincides with the loss of promoter occupancy in vivo.
Article in The EMBO journal, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 101 citations in OpenAlex.
- An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.International journal of molecular sciences · 2024Article
- Long range inter-chromosomal interaction of Oct4 distal enhancer loci regulates ESCs pluripotency.Cell death discovery · 2023Article
- The functional diversity of the POUV-class proteins across vertebrates.Open biology · 2022Review
- An Overview on the Complexity of OCT4: at the Level of DNA, RNA and Protein.Stem cell reviews and reports · 2021Review
- Cruciform Formable Sequences withinInternational journal of molecular sciences · 2021Article
- Identification and Characterization of theStem cells international · 2019Article
- A distinct isoform of ZNF207 controls self-renewal and pluripotency of human embryonic stem cells.Nature communications · 2018Article
- Evolutionary Conservation of pou5f3 Genomic Organization and Its Dynamic Distribution during Embryogenesis and in Adult Gonads in Japanese Flounder Paralichthys olivaceus.International journal of molecular sciences · 2017Article
- Mechanisms Regulating Stemness and Differentiation in Embryonal Carcinoma Cells.Stem cells international · 2017Review
- Reprogramming to pluripotency using designer TALE transcription factors targeting enhancers.Stem cell reports · 2013Article
- Nuclear orphan receptor TLX induces Oct-3/4 for the survival and maintenance of adult hippocampal progenitors upon hypoxia.The Journal of biological chemistry · 2011Article
- Retinoic acid accelerates downregulation of the Xist repressor, Oct4, and increases the likelihood of Xist activation when Tsix is deficient.BMC developmental biology · 2010Article
- Cloning and Characterization of 3.1kb Promoter Region of the Oct4 Gene from the Fischer 344 Rat.The open stem cell journal · 2009Article
- Novel variants of Oct-3/4 gene expressed in mouse somatic cells.The Journal of biological chemistry · 2008Article
- Neural differentiation arrest in embryonal carcinoma cells with forced expression of EWS-FLI1.Journal of neuro-oncology · 2008Article
- Ubiquitin E3 ligase Ring1b/Rnf2 of polycomb repressive complex 1 contributes to stable maintenance of mouse embryonic stem cells.PloS one · 2008Article
- Structure and expression pattern of Oct4 gene are conserved in vole Microtus rossiaemeridionalis.BMC genomics · 2008Article
- Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract.Molecular biology of the cell · 2007Article
- Differentiation of murine embryonic stem cells induces progesterone receptor gene expression.Experimental cell research · 2005Article
- Orphan nuclear receptor LRH-1 is required to maintain Oct4 expression at the epiblast stage of embryonic development.Molecular and cellular biology · 2005Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Oct3/4, a hallmark of the earliest stages of embryogenesis, is expressed in undifferentiated embryonal carcinoma (EC) and embryonic stem (ES) cells. Oct3/4 gene expression is dependent on the promoter region, the proximal enhancer and the newly identified distal enhancer. We have analysed in vivo occupancy of these elements. In undifferentiated EC and ES cells, strong footprints were detected at specific sites of all three regulatory elements. These were promptly lost upon RA treatment in ES cells and in P19 EC cells, in parallel with sharply reduced Oct3/4 mRNA levels. Thus, the occupancy of regulatory elements is coupled with Oct3/4 expression, and RA treatment causes coordinated factor displacement, leading to extinction of gene activity. In F9 EC cells, footprint was first abolished at the proximal enhancer. However, this loss of binding site occupancy did not result in a decrease in Oct3/4 mRNA levels. The partial factor displacement seen in F9 EC cells, combined with the observation that EC and ES cells utilize the proximal and distal enhancers in differential manner, indicate the complex pattern of Oct3/4 gene regulation, which could reflect a cell type- and lineage-specific expression of the gene in vivo.
Indexed as
Identifiers
8631309PMC450286W171108017What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.