ArticleNucleic acids research1996
Age-dependent silencing of globin transgenes in the mouse.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 93 citations in OpenAlex.
- Overexpression of soluble epoxide hydrolase reduces post-ischemic recovery of cardiac contractile function.Biochemical pharmacology · 2024Article
- Generation of Transgenic Fluorescent Reporter Lines for Studying Hematopoietic Development in the Mouse.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Reduced PRC2 function alters male germline epigenetic programming and paternal inheritance.BMC biology · 2018Article
- Optogenetic Peripheral Nerve Immunogenicity.Scientific reports · 2018Article
- Swine PPAR-γ2 expression upregulated in skeletal muscle of transgenic mice via the swine Myozenin-1 gene promoter.Transgenic research · 2015Article
- Suppression of oxidative phosphorylation in mouse embryonic fibroblast cells deficient in apurinic/apyrimidinic endonuclease.DNA repair · 2015Article
- Generation of transgenic mouse fluorescent reporter lines for studying hematopoietic development.Methods in molecular biology (Clifton, N.J.) · 2014Article
- The use of mouse models to study epigenetics.Cold Spring Harbor perspectives in biology · 2013Review
- Postnatal development- and age-related changes in DNA-methylation patterns in the human genome.Nucleic acids research · 2012Article
- Postnatal liver growth and regeneration are independent of c-myc in a mouse model of conditional hepatic c-myc deletion.BMC physiology · 2012Article
- Effect of trichostatin A and 5-Aza-2'-deoxycytidine on transgene reactivation and epigenetic modification in transgenic pig fibroblast cells.Molecular and cellular biochemistry · 2011Article
- Establishment of a pig fibroblast-derived cell line for locus-directed transgene expression in cell cultures and blastocysts.Molecular biology reports · 2011Article
- Laser-evoked synaptic transmission in cultured hippocampal neurons expressing channelrhodopsin-2 delivered by adeno-associated virus.Journal of neuroscience methods · 2009Article
- Transgene expression is associated with copy number and cytomegalovirus promoter methylation in transgenic pigs.PloS one · 2009Article
- Article
- Increased p53 activity does not accelerate telomere-driven ageing.EMBO reports · 2006Article
- Making better transgenic models: conditional, temporal, and spatial approaches.Molecular biotechnology · 2005Review
- Stem cell plasticity: a rare cell, not a rare event.Stem cell reviews · 2005Review
- Transgene methylation in mice reflects copy number but not expression level.Molecular biotechnology · 2004Article
- Mono-allelic expression of variegating transgene locus in the mouse.Transgenic research · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Variegation of transgene expression, a heterocellular or mosaic pattern of expression seen in all mice in a given transgenic line, is a frequently observed but unexplained phenomenon. We have encountered variegation with globin transgenes; when lacZ expression is driven by globin control elements a proportion of erythrocytes express beta-galactosidase (beta-gal), while the remaining erythrocytes express none. The percentage of expressing cells is constant within each line (at any particular developmental stage), but varies between lines. Such variation may account for much of the line-to-line variability which has been reported in the expression of a transgene construct. We have now extended these observations by studying expression of several globin/lacZ transgenes with increasing age. Expression of beta-gal is variegated in all lines in adult mice, including those made with a beta-globin promoter and locus control region driving lacZ. The extent of variegation differs widely between lines, but in all lines there is a marked decline in the number of erythrocytes expressing beta-gal with increasing age. Progression of silencing continues long past the point at which globin switching is complete, suggesting that it is not related to this process. We observe that age-dependent silencing is most severe in high copy number animals. Increasing variegation of transgene expression with ageing of mice is likely to complicate interpretation of the developmental regulation of transgenes. We speculate that it reflects a general mechanism of epigenetic regulation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.