ArticleNucleic acids research1996
Multiplex messenger assay: simultaneous, quantitative measurement of expression of many genes in the context of T cell activation.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 80 citations in OpenAlex.
- Identification of woolliness response genes in peach fruit after post-harvest treatments.Journal of experimental botany · 2008Article
- Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression.Plant physiology · 2005Article
- Expression profiling with arrays of randomly disrupted genes in mouse embryonic stem cells leads to in vivo functional analysis.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Improving DNA array data quality by minimising 'neighbourhood' effects.Nucleic acids research · 2002Article
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- A systematic molecular genetic approach to study mammalian germline development.The International journal of developmental biology · 1998Review
- Adaptor-tagged competitive PCR: a novel method for measuring relative gene expression.Nucleic acids research · 1997Article
- Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The hybridization signature approach, using colony filters and labeled complex probes, can provide high throughput measurement of gene activity. We describe here the implementation of this method to follow the expression levels of 47 genes in resting and activated T cells, as well as in epithelial cells. Using 4-fold spotting of colonies, imaging plate detection and various correction and normalization procedures, the technique is sensitive enough to quantify expression levels for sequences present at 0.005% abundance in the probe. Comparison with Northern blotting shows good consistency between the two methods. Upon activation of a T cell clone by an anti-CD3 antibody variations ranging from 2- to 20-fold are measured, some of which had not been reported previously. This 'multiplex messenger assay' method, performed using available commercial apparatus, can be used in many cases where simultaneous assessment of mRNA levels for many genes is of interest.
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