ArticleNucleic acids research1996
Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 63 citations in OpenAlex.
- Photocleavable Mass-Tagged Oligonucleotide Probes for Multiplexed and Multiomic Tissue Imaging of Targeted Transcripts.Journal of the American Society for Mass Spectrometry · 2025Article
- MALDI HiPLEX-IHC: multiomic and multimodal imaging of targeted intact proteins in tissues.Frontiers in chemistry · 2023Article
- Studies on Transcriptional Incorporation of 5'-N-Triphosphates of 5'-Amino-5'-Deoxyribonucleosides.PloS one · 2016Article
- Highly parallel oligonucleotide purification and functionalization using reversible chemistry.Nucleic acids research · 2012Article
- Photocleavage-based affinity purification and printing of cell-free expressed proteins: application to proteome microarrays.Analytical biochemistry · 2008Article
- Analytical biochemistry of DNA--protein assemblies from crude cell extracts.Nucleic acids research · 2007Article
- Design and synthesis of a photocleavable biotinylated nucleotide for DNA analysis by mass spectrometry.Nucleic acids research · 2004Article
- Photocleavage of a 2-nitrobenzyl linker bridging a fluorophore to the 5' end of DNA.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Fluoride-cleavable biotinylation phosphoramidite for 5'-end-labeling and affinity purification of synthetic oligonucleotides.Nucleic acids research · 2003Article
- Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.Nucleic acids research · 1997Article
- Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) of endonuclease digests of RNA.Nucleic acids research · 1997Article
- Biotinylated photocleavable polyethylenimine: capture and triggered release of nucleic acids from solid supports.Bioconjugate chemistryArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report the design, synthesis and evaluation of a non-nucleosidic photocleavable biotin phosphoramidite (PCB-phosphoramidite) which provides a simple method for purification and phosphorylation of oligonucleotides. This reagent introduces a photocleavable biotin label (PCB) on the 5'-terminal phosphate of synthetic oligonucleotides and is fully compatible with automated solid support synthesis. HPLC analysis shows that the PCB moiety is introduced predominantly on full-length sequences and is retained during cleavage of the synthetic oligonucleotide from the solid support and during subsequent deprotection with ammonia. The full-length 5-PCB-labeled oligonucleotide can then be selectively isolated from the crude oligonucleotide mixture by incubation with immobilized streptavidin. Upon irradiation with 300-350 nm light the 5'-PCB moiety is cleaved with high efficiency in <4 min, resulting in rapid release of affinity-purified 5'-phosphorylated oligonucleotides into solution. 5'-PCB-labeled oligonucleotides should be useful in a variety of applications in molecular biology, including cassette mutagenesis and PCR. As an example, PCB-phosphoramidite has been used for the synthesis, purification and phosphorylation of 50-and 60mer oligonucleotides.
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