ArticleNucleic acids research1996
Restriction landmark cDNA scanning (RLCS): a novel cDNA display system using two-dimensional gel electrophoresis.
Article in Nucleic acids research, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed, 33 citations in OpenAlex.
- Prefrontal-enriched SLIT1 expression in Old World monkey cortex established during the postnatal development.Cerebral cortex (New York, N.Y. : 1991) · 2010Article
- Paraneoplastic antigen-like 5 gene (PNMA5) is preferentially expressed in the association areas in a primate specific manner.Cerebral cortex (New York, N.Y. : 1991) · 2009Article
- Enriched expression of serotonin 1B and 2A receptor genes in macaque visual cortex and their bidirectional modulatory effects on neuronal responses.Cerebral cortex (New York, N.Y. : 1991) · 2009Article
- Differential expression patterns of occ1-related genes in adult monkey visual cortex.Cerebral cortex (New York, N.Y. : 1991) · 2009Article
- Article
- Multiple isoform recovery (MIR)-PCR: a simple method for the isolation of related mRNA isoforms.Nucleic acids research · 1998Article
- Ordered differential display: a simple method for systematic comparison of gene expression profiles.Nucleic acids research · 1997Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a new method, designated restriction landmark cDNA scanning (RLCS), which displays many cDNA species quantitatively and simultaneously as two-dimensional gel spots. In this method cDNA species of uniform length were prepared for each mRNA species using restriction enzymes. After the restriction enzyme sites were radiolabeled as landmarks, the labeled fragments were subjected to high resolution two-dimensional gel electrophoresis. In analyses of cDNA samples from adult mouse liver and brain (cerebral cortex, cerebellum and brain stem) we detected approximately 500 and >1000 discrete gel spots respectively of various intensities at a time. The spot patterns of the three brain regions were very similar, although not identical, but were quite different from the pattern for the liver. RNA blot hybridization analysis using several cloned spot DNAs as probes showed that differences in intensity of the spots among RLCS profiles correlated well with expression levels of the corresponding mRNA species in the brain regions. Because the spots and their intensities reflect distinct mRNA species and their expression level respectively, the RLCS is a novel cDNA display system which provides a great deal of information and should be useful for systematic documentation of differentially expressed genes.
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