ArticleMolecular and cellular biology1996
Tethered Sir3p nucleates silencing at telomeres and internal loci in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 73 citations in OpenAlex.
- Context-dependent function of the transcriptional regulator Rap1 in gene silencing and activation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Context dependent function of the transcriptional regulator Rap1 in gene silencing and activation inbioRxiv : the preprint server for biology · 2023Article
- Regulation of Antigenic Variation byPathogens (Basel, Switzerland) · 2021Review
- Keeping Balance Between Genetic Stability and Plasticity at the Telomere and Subtelomere ofFrontiers in cell and developmental biology · 2021Review
- Chromatin regulation at the frontier of synthetic biology.Nature reviews. Genetics · 2015Review
- Article
- Dot1 binding induces chromatin rearrangements by histone methylation-dependent and -independent mechanisms.Epigenetics & chromatin · 2011Article
- Saccharomyces cerevisiae Esc2p interacts with Sir2p through a small ubiquitin-like modifier (SUMO)-binding motif and regulates transcriptionally silent chromatin in a locus-dependent manner.The Journal of biological chemistry · 2010Article
- Silent information regulator 3: the Goldilocks of the silencing complex.Genes & development · 2010Review
- Inactivation of the Sas2 histone acetyltransferase delays senescence driven by telomere dysfunction.The EMBO journal · 2010Article
- The functional importance of telomere clustering: global changes in gene expression result from SIR factor dispersion.Genome research · 2009Article
- Conversion of a replication origin to a silencer through a pathway shared by a Forkhead transcription factor and an S phase cyclin.Molecular biology of the cell · 2008Article
- Sir3 C-terminal domain involvement in the initiation and spreading of heterochromatin.Molecular and cellular biology · 2006Article
- Mcm10 is required for the maintenance of transcriptional silencing in Saccharomyces cerevisiae.Genetics · 2005Article
- Barrier proteins remodel and modify chromatin to restrict silenced domains.Molecular and cellular biology · 2004Article
- One-hybrid screens at the Saccharomyces cerevisiae HMR locus identify novel transcriptional silencing factors.Genetics · 2004Article
- The Rap1p-telomere complex does not determine the replicative capacity of telomerase-deficient yeast.Molecular and cellular biology · 2003Article
- Targeting assay to study the cis functions of human telomeric proteins: evidence for inhibition of telomerase by TRF1 and for activation of telomere degradation by TRF2.Molecular and cellular biology · 2002Article
- Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe.Nucleic acids research · 2002Review
- Multiple interactions in Sir protein recruitment by Rap1p at silencers and telomeres in yeast.Molecular and cellular biology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Rap1p binds to sites embedded within the Saccharomyces cerevisiae telomeric TG1-3 tract. Previous studies have led to the hypothesis that Rap1p may recruit Sir3p and Sir3p-associating factors to the telomere. To test this, we tethered Sir3p adjacent to the telomere via LexA binding sites in the rap1-17 mutant that truncates the Rap1p C-terminal 165 amino acids thought to contain sites for Sir3p association. Tethering of LexA-Sir3p adjacent to the telomere is sufficient to restore telomeric silencing, indicating that Sir3p can nucleate silencing at the telomere. Tethering of LexA-Sir3p or the LexA-Sir3p(N2O5) gain-of-function protein to a telomeric LexA site hyperrepresses an adjacent ADE2 gene in wild-type cells. Hence, Sir3p recruitment to the telomere is limiting in telomeric silencing. In addition, LexA-Sir3p(N2O5) hyperrepresses telomeric silencing when tethered to a subtelomeric site 3.6 kb from the telomeric tract. This hyperrepression is dependent on the C terminus of Rap1p, suggesting that subtelomeric LexA-Sir3p(N205) can interact with Rap1p-associated factors at the telomere. We also demonstrate that LexA-Sir3p or LexA-Sir3p(N205) tethered in cis with a short tract of telomeric TG1-3 sequences is sufficient to confer silencing at an internal chromosomal position. Internal silencing is enhanced in rap1-17 strains. We propose that sequestration of silencing factors at the telomere limits the efficiency of internal silencing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.