ArticleMolecular and cellular biology1996
Transcription of the dominant-negative helix-loop-helix protein Id1 is regulated by a protein complex containing the immediate-early response gene Egr-1.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 122 citations in OpenAlex.
- Dominant and recessive ATOH1 variants cause distinct neurodevelopmental disorders with hearing loss.American journal of human genetics · 2026Article
- Id proteins: emerging roles in CNS disease and targets for modifying neural stemcell behavior.Cell and tissue research · 2022Review
- Anti-tumor effects of an ID antagonist with no observed acquired resistance.NPJ breast cancer · 2021Article
- Inhibitor of Differentiation 1 (Id1) in Cancer and Cancer Therapy.International journal of medical sciences · 2020Review
- LEF1/Id3/HRAS axis promotes the tumorigenesis and progression of esophageal squamous cell carcinoma.International journal of biological sciences · 2020Article
- A Small-Molecule Pan-Id Antagonist Inhibits Pathologic Ocular Neovascularization.Cell reports · 2019Article
- Inhibitor of DNA binding proteins: implications in human cancer progression and metastasis.American journal of translational research · 2018Review
- Role of ID Proteins in BMP4 Inhibition of Profibrotic Effects of TGF-β2 in Human TM Cells.Investigative ophthalmology & visual science · 2017Article
- The Id-protein family in developmental and cancer-associated pathways.Cell communication and signaling : CCS · 2017Review
- Elk-3 Contributes to the Progression of Liver Fibrosis by Regulating the Epithelial-Mesenchymal Transition.Gut and liver · 2017Article
- Cox-2-derived PGE2 induces Id1-dependent radiation resistance and self-renewal in experimental glioblastoma.Neuro-oncology · 2016Article
- Inhibition of BMP and of TGFβ receptors downregulates expression of XIAP and TAK1 leading to lung cancer cell death.Molecular cancer · 2016Article
- Id1 expression in endothelial cells of the colon is required for normal response to injury.The American journal of pathology · 2015Article
- Synergistic interaction between the fibroblast growth factor and bone morphogenetic protein signaling pathways in lens cells.Molecular biology of the cell · 2015Article
- Id1 Deficiency Protects against Tumor Formation in Apc(Min/+) Mice but Not in a Mouse Model of Colitis-Associated Colon Cancer.Cancer prevention research (Philadelphia, Pa.) · 2015Article
- LIF negatively regulates tumour-suppressor p53 through Stat3/ID1/MDM2 in colorectal cancers.Nature communications · 2014Article
- The ID proteins: master regulators of cancer stem cells and tumour aggressiveness.Nature reviews. Cancer · 2014Review
- Androgen deprivation therapy induces androgen receptor-dependent upregulation of Egr1 in prostate cancers.International journal of clinical and experimental pathology · 2014Article
- Serum regulation of Id1 expression by a BMP pathway and BMP responsive element.Biochimica et biophysica acta · 2013Article
- DPY30 regulates pathways in cellular senescence through ID protein expression.The EMBO journal · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The expression of Id1, a helix-loop-helix protein which inhibits the activity of basic helix-loop-helix transcription factors, is down-regulated during cellular differentiation and cell cycle withdrawal both in tissue culture models and in mouse embryos. In order to study the mechanism of control of Idl expression, we have isolated a 210-bp enhancer element in the upstream region of the Id1 gene whose activity recapitulates Id1 expression in C2C12 muscle cells and C3H10T1/2 fibroblasts: i.e., this element is active in proliferating cells in the presence of serum and completely inactivated upon mitogen depletion, cell cycle withdrawal, and (in the case of C2C12) induced myoblast differentiation. Using linker-scanning mutations and site-directed mutagenesis in transient transfection experiments, we have identified two functional elements within the 210-bp enhancer which are required for proper serum responsiveness. One element (A) contains a consensus Egr-1 binding site and additional flanking sequences required for optimal activity, and the other element (B) fits no known consensus. Gel shift experiments demonstrate that the protein complex binding to the A site contains Egr-1 and other proteins. This complex as well as a protein complex that binds to the B site is lost within 24 h of serum depletion, correlating with the down-regulation of Id1 expression. On the basis of these findings, we propose that the regulation of the Id1 response to serum is mediated in part by the early response gene Egr-1 and as such provides a signaling link between the early-growth-response transcription factors and dominant-negative helix-loop-helix proteins.
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