ArticleMolecular and cellular biology1996
Common core sequences are found in skeletal muscle slow- and fast-fiber-type-specific regulatory elements.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 77 citations in OpenAlex.
- Ets2 Is an Activity-Dependent Transcriptional Regulator of a Fast-Twitch Muscle Program.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation.Scientific reports · 2021Article
- The LIM domain protein nTRIP6 acts as a co-repressor for the transcription factor MEF2C in myoblasts.Scientific reports · 2016Article
- DNA methylation regulates mouse cardiac myofibril gene expression during heart development.Journal of biomedical science · 2015Article
- Myocyte enhancer factor 2C function in skeletal muscle is required for normal growth and glucose metabolism in mice.Skeletal muscle · 2015Article
- Nitric oxide synthase inhibition prevents activity-induced calcineurin-NFATc1 signalling and fast-to-slow skeletal muscle fibre type conversions.The Journal of physiology · 2012Article
- Genome-wide mapping of Sox6 binding sites in skeletal muscle reveals both direct and indirect regulation of muscle terminal differentiation by Sox6.BMC developmental biology · 2011Article
- Excitation-transcription coupling in skeletal muscle: the molecular pathways of exercise.Biological reviews of the Cambridge Philosophical Society · 2011Review
- The ups and downs of gene regulation by electrical activity in skeletal muscles.Journal of muscle research and cell motility · 2009Review
- Activity-dependent repression of muscle genes by NFAT.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Calcineurin A and CaMKIV transactivate PGC-1alpha promoter, but differentially regulate cytochrome c promoter in rat skeletal muscle.Pflugers Archiv : European journal of physiology · 2007Article
- Imaging transcription in vivo: distinct regulatory effects of fast and slow activity patterns on promoter elements from vertebrate troponin I isoform genes.The Journal of physiology · 2005Article
- Structure and regulation of human troponin genes.Molecular and cellular biochemistry · 2004Article
- Transcription of Drosophila troponin I gene is regulated by two conserved, functionally identical, synergistic elements.Molecular biology of the cell · 2004Article
- Molecular dissection of DNA sequences and factors involved in slow muscle-specific transcription.Molecular and cellular biology · 2001Article
- MEF2 responds to multiple calcium-regulated signals in the control of skeletal muscle fiber type.The EMBO journal · 2000Article
- Article
- Fiber-type-specific transcription of the troponin I slow gene is regulated by multiple elements.Molecular and cellular biology · 1999Article
- Identification of a novel slow-muscle-fiber enhancer binding protein, MusTRD1.Molecular and cellular biology · 1998Article
- A calcineurin-dependent transcriptional pathway controls skeletal muscle fiber type.Genes & development · 1998Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The molecular mechanisms generating muscle diversity during development are unknown. The phenotypic properties of slow- and fast-twitch myofibers are determined by the selective transcription of genes coding for contractile proteins and metabolic enzymes in these muscles, properties that fail to develop in cultured muscle. Using transgenic mice, we have identified regulatory elements in the evolutionarily related troponin slow (TnIs) and fast (TnIf) genes that confer specific transcription in either slow or fast muscles. Analysis of serial deletions of the rat TnIs upstream region revealed that sequences between kb -0.95 and -0.5 are necessary to confer slow-fiber-specific transcription; the -0.5-kb fragment containing the basal promoter was inactive in five transgenic mouse lines tested. We identified a 128-bp regulatory element residing at kb -0.8 that, when linked to the -0.5-kb TnIs promoter, specifically confers transcription to slow-twitch muscles. To identify sequences directing fast-fiber-specific transcription, we generated transgenic mice harboring a construct containing the TnIs kb -0.5 promoter fused to a 144-bp enhancer derived from the quail TnIf gene. Mice harboring the TnIf/TnIs chimera construct expressed the transgene in fast but not in slow muscles, indicating that these regulatory elements are sufficient to confer fiber-type-specific transcription. Alignment of rat TnIs and quail TnIf regulatory sequences indicates that there is a conserved spatial organization of core elements, namely, an E box, a CCAC box, a MEF-2-like sequence, and a previously uncharacterized motif. The core elements were shown to bind their cognate factors by electrophoretic mobility shift assays, and their mutation demonstrated that the TnIs CCAC and E boxes are necessary for transgene expression. Our results suggest that the interaction of closely related transcriptional protein-DNA complexes is utilized to specify fiber type diversity.
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