ArticleMolecular and cellular biology1996
Evidence for posttranscriptional regulation of C/EBPalpha and C/EBPbeta isoform expression during the lipopolysaccharide-mediated acute-phase response.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 156 citations in OpenAlex.
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- Inherited Predispositions to Myeloid Neoplasms: Pathogenesis and Clinical Implications.Annual review of pathology · 2025Review
- The Role of CYP3A in Health and Disease.Biomedicines · 2022Review
- At the Crossroads of the Adipocyte and Osteoclast Differentiation Programs: Future Therapeutic Perspectives.International journal of molecular sciences · 2020Review
- Human Papillomavirus Infection in Head and Neck Squamous Cell Carcinomas: Transcriptional Triggers and Changed Disease Patterns.Frontiers in cellular and infection microbiology · 2020Review
- Regulation of drug-metabolizing enzymes in infectious and inflammatory disease: implications for biologics-small molecule drug interactions.Expert opinion on drug metabolism & toxicology · 2017Review
- Non-steady-state hematopoiesis regulated by the C/EBPβ transcription factor.Cancer science · 2015Article
- Orosomucoid 2 inhibits tumor metastasis and is upregulated by CCAAT/enhancer binding protein β in hepatocellular carcinomas.Oncotarget · 2015Article
- CCAAT/enhancer binding protein β in relation to ER stress, inflammation, and metabolic disturbances.BioMed research international · 2015Review
- CCAAT-enhancer binding protein-β expression and elevation in Alzheimer's disease and microglial cell cultures.PloS one · 2014Article
- Identification of alternatively translated Tetherin isoforms with differing antiviral and signaling activities.PLoS pathogens · 2012Article
- LIP expression is regulated by IGF-1R signaling and participates in suppression of anoikis.Molecular cancer · 2011Article
- C/EBPβ-Thr217 phosphorylation signaling contributes to the development of lung injury and fibrosis in mice.PloS one · 2011Article
- A proteomics analysis of the effects of chronic hemiparetic stroke on troponin T expression in human vastus lateralis.The journals of gerontology. Series A, Biological sciences and medical sciences · 2009Article
- Article
- CCAAT/enhancer-binding protein beta: its role in breast cancer and associations with receptor tyrosine kinases.Expert reviews in molecular medicine · 2009Review
- St. John's Wort protein, p27SJ, regulates the MCP-1 promoter.Molecular immunology · 2008Article
- Age-related alterations in oxidatively damaged proteins of mouse heart mitochondrial electron transport chain complexes.Free radical biology & medicine · 2008Article
- C/EBPbeta induces chromatin opening at a cell-type-specific enhancer.Molecular and cellular biology · 2008Article
- Age-related increases in oxidatively damaged proteins of mouse kidney mitochondrial electron transport chain complexes.Free radical biology & medicine · 2007Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The mRNAs of the CCAAT/enhancer-binding trans-activator proteins (C/EBPalpha and C/EBPbeta) serve as templates for the differential translation of several isoforms which have specific transcriptional regulatory functions. By using an oligonucleotide corresponding to the C/EBP binding site of the mouse alpha1-acid glycoprotein promoter, we detected multiple forms of C/EBPalpha and C/EBP++ beta proteins in the mouse liver that have DNA-binding activity. By using specific antisera, we detected C/EBPalphas with molecular masses of 42, 38, 30, and 20 kDa that have DNA-binding activity. The pool levels of the 42- and 30-kDa isoforms were high in control nuclear extracts and decreased significantly after lipopolysaccharide (LPS) treatment. The binding activity and protein levels of the 20-kDa isoform are low in controls and increase dramatically after LPS treatment. C/EBPbeta isoforms with molecular masses of 35, 20, and 16 kDa were also detected. The 35-kDa pool level did not change whereas the 20-kDa isoform was strongly induced in response to LPS. Western (immunoblot) and Southwestern (DNA-protein) analyses show that p42 C/EBPalpha forms specific complexes with the alpha1-acid glycoprotein oligonucleotide in control nuclear extract and that p20 C/EBP beta forms complexes in LPS-treated liver. Our studies suggest that synthesis of specific C/EBPalpha and C/EBPbeta isoforms occurred in the normal liver in vivo and that LPS mediated a differential initiation and inhibition of translation at specific AUG sites within each mRNA. The qualitative and quantitative changes in C/EBPalpha and C/EBPbeta isoform pool levels suggest that LPS or an LPS-stimulated factor can regulate the selection of AUG start sites for both activation and repression of translation. This regulation appears to involve an LPS-mediated down-regulation of initiation at the first AUG codon of the 42-kDa C/EBPalpha and dramatic translational up-regulation at the fifth AUG codon of the 20-kDa C/EBPalpha and the third AUG codon of the 20-kDa C/EBPbeta. These regulatory events suggest the existence of proteins that may act as translational trans-acting factors.
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