ArticleMolecular and cellular biology1996
The yeast alpha2 and Mcm1 proteins interact through a region similar to a motif found in homeodomain proteins of higher eukaryotes.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Ancient transcriptional regulators can easily evolve new pair-wise cooperativity.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- DNA binding by the MATα2 transcription factor controls its access to alternative ubiquitin-modification pathways.Molecular biology of the cell · 2018Article
- STUbL-mediated degradation of the transcription factor MATα2 requires degradation elements that coincide with corepressor binding sites.Molecular biology of the cell · 2015Article
- Article
- SUMO-independent in vivo activity of a SUMO-targeted ubiquitin ligase toward a short-lived transcription factor.Genes & development · 2010Article
- A competitive transcription factor binding mechanism determines the timing of late cell cycle-dependent gene expression.Molecular cell · 2010Article
- Identification of cell cycle-related regulatory motifs using a kernel canonical correlation analysis.BMC genomics · 2009Article
- MAL and ternary complex factor use different mechanisms to contact a common surface on the serum response factor DNA-binding domain.Molecular and cellular biology · 2006Article
- The Arabidopsis zinc finger-homeodomain genes encode proteins with unique biochemical properties that are coordinately expressed during floral development.Plant physiology · 2006Article
- Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast.PLoS biology · 2004Article
- Alpha1-induced DNA bending is required for transcriptional activation by the Mcm1-alpha1 complex.Nucleic acids research · 2004Article
- Repression of the yeast HO gene by the MATalpha2 and MATa1 homeodomain proteins.Nucleic acids research · 2004Article
- Mcm1p-induced DNA bending regulates the formation of ternary transcription factor complexes.Molecular and cellular biology · 2003Article
- Interactions of the Mcm1 MADS box protein with cofactors that regulate mating in yeast.Molecular and cellular biology · 2002Article
- The mapping of nucleosomes and regulatory protein binding sites at the Saccharomyces cerevisiae MFA2 gene: a high resolution approach.Nucleic acids research · 2001Article
- Tup1p represses Mcm1p transcriptional activation and chromatin remodeling of an a-cell-specific gene.The EMBO journal · 2000Article
- A sequence resembling a peroxisomal targeting sequence directs the interaction between the tetratricopeptide repeats of Ssn6 and the homeodomain of alpha 2.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Scanning mutagenesis of Mcm1: residues required for DNA binding, DNA bending, and transcriptional activation by a MADS-box protein.Molecular and cellular biology · 2000Article
- Homeodomain-DNA interactions of the Pho2 protein are promoter-dependent.Nucleic acids research · 1997Article
- Alpha2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III.Genes & development · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Homeodomain proteins are transcriptional regulatory factors that, in general, bind DNA with relatively low sequence specificity and affinity. One mechanism homeodomain proteins use to increase their biological specificity is through interactions with other DNA-binding proteins. We have examined how the yeast (Saccharomyces cerevisiae) homeodomain protein alpha2 specifically interacts with Mcm1, a MADS box protein, to bind DNA specifically and repress transcription. A patch of predominantly hydrophobic residues within a region preceding the homeodomain of alpha2 has been identified that specifies direct interaction with Mcm1 in the absence of DNA. This hydrophobic patch is required for cooperative DNA binding with Mcm1 in vitro and for transcriptional repression in vivo. We have also found that a conserved motif, termed YPWM, frequently found in homeodomain proteins of insects and mammals, partially functions in place of the patch in alpha2 to interact with Mcm1. These findings suggest that homeodomain proteins from diverse organisms may use analogous interaction motifs to associate with other proteins to achieve high levels of DNA binding affinity and specificity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.