ArticleMolecular and cellular biology1996
A role for a bent DNA structure in E2F-mediated transcription activation.
Article in Molecular and cellular biology, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 65 citations in OpenAlex.
- Nuclear Respiratory Factor-1, a Novel SMAD4 Binding Protein, Represses TGF-β/SMAD4 Signaling by Functioning as a Transcriptional Cofactor.International journal of molecular sciences · 2021Article
- Differential requirement for dimerization partner DP between E2F-dependent activation of tumor suppressor and growth-related genes.Scientific reports · 2018Article
- DNA virus replication compartments.Journal of virology · 2014Review
- An appraisal of human mitochondrial DNA instability: new insights into the role of non-canonical DNA structures and sequence motifs.PloS one · 2013Article
- JAZ mediates G1 cell cycle arrest by interacting with and inhibiting E2F1.Cell cycle (Georgetown, Tex.) · 2011Article
- Genome-wide analysis of transcription factor E2F1 mutant proteins reveals that N- and C-terminal protein interaction domains do not participate in targeting E2F1 to the human genome.The Journal of biological chemistry · 2011Article
- CDKN1C negatively regulates RNA polymerase II C-terminal domain phosphorylation in an E2F1-dependent manner.The Journal of biological chemistry · 2010Article
- EBP1 regulates organ size through cell growth and proliferation in plants.The EMBO journal · 2006Article
- The ErbB3 binding protein Ebp1 interacts with Sin3A to repress E2F1 and AR-mediated transcription.Nucleic acids research · 2005Article
- Left-handedly curved DNA regulates accessibility to cis-DNA elements in chromatin.Nucleic acids research · 2003Article
- Differences in DNA binding properties between E2F1 and E2F4 specify repression of the Mcl-1 promoter.Oncogene · 2002Article
- Adenovirus early E4 genes in viral oncogenesis.Oncogene · 2001Review
- Identification of E2F-3B, an alternative form of E2F-3 lacking a conserved N-terminal region.Oncogene · 2000Article
- Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes.Oncogene · 1999Article
- Distinct frequency-distributions of homopolymeric DNA tracts in different genomes.Nucleic acids research · 1998Article
- Subunit composition determines E2F DNA-binding site specificity.Molecular and cellular biology · 1997Article
- DNA binding by MADS-box transcription factors: a molecular mechanism for differential DNA bending.Molecular and cellular biology · 1997Article
- Position-dependent transcriptional regulation of the murine dihydrofolate reductase promoter by the E2F transactivation domain.Molecular and cellular biology · 1997Article
- Targeting E2F1-DNA complexes with microgonotropen DNA binding agents.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined the role of promoter architecture, as well as that of the DNA-bending capacity of the E2F transcription factor family, in the activation of transcription. DNA phasing analysis revealed that a consensus E2F site in the E2F1 promoter possesses an inherent bend with a net magnitude of 40 +/-2 degrees and with an orientation toward the major groove relative to the center of the E2F site. The inherent DNA bend is reversed upon binding of E2F, generating a net bend with a magnitude of 25 +/- 3 degrees oriented toward the minor groove relative to the center of the E2F site. We also found that three members of the E2F family, in conjunction with the DP1 protein, bend the DNA toward the minor groove, suggesting that DNA bending is a characteristic of the entire E2F family. The Rb-E2F complex, on the other hand, does not reverse the intrinsic DNA bend. Analysis of a series of E2F1 deletion mutants defined E2F1 sequences which are not required for DNA binding but are necessary for the DNA-bending capacity of E2F. An internal region of E2F1, previously termed the marked box, which is highly homologous among E2F family members, was particularly important in DNA bending. We also found that a bent DNA structure can be a contributory component in the activation of the E2F1 promoter but is not critical in the repression of that promoter in quiescent cells. This finding suggests that E2F exhibits characteristics typical of modular transcription factors, with independent DNA-binding and transcriptional activation functions, but also has features of architectural factors that alter DNA structure.
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